Cytoskeletal Signaling

Cellular Localization IF Antibody Sampler Kit

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4753 の推奨プロトコール最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。注：各製品に最適化されたプロトコールをリンクしています。

キット内容	容量	用途	種交差性	検出タンパク質の分子量	Isotype
β-Tubulin (9F3) Rabbit mAb #2128	40 µl	W IHC-P IF-IC F	H M R Mk Z B (C)	55	Rabbit IgG
COX IV (3E11) Rabbit mAb #4850	40 µl	W IP IHC-P IHC-F IF-IC F	H R Mk Z B Pg	17	Rabbit IgG
NUP98 (C39A3) Rabbit mAb #2598	40 µl	W IP IF-IC	H M R Mk	98	Rabbit IgG
CENP-A Antibody #2186	40 µl	W IF-IC	H	17	Rabbit
Fibrillarin (C13C3) Rabbit mAb #2639	40 µl	W IF-IC	H M R Mk	37	Rabbit IgG
LC3B (D11) XP^® Rabbit mAb #3868	40 µl	W IP IHC-P IF-IC F	H M R (Mk) (B) (Pg)	14, 16	Rabbit IgG
Rab5 (C8B1) Rabbit mAb #3547	40 µl	W IF-IC	H M R Mk	25	Rabbit IgG
Calnexin (C5C9) Rabbit mAb #2679	40 µl	W IHC-P IF-IC	H Mk	90	Rabbit
Histone H3 (D1H2) XP^® Rabbit mAb #4499	40 µl	W IHC-P IF-IC	H M R Mk (Hm) (C) (Dm) (X) (Z) (B)	17	Rabbit IgG

※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。

社内データ

IF-IC

Confocal immunofluorescent analysis of HeLa cells using β-Tubulin (9F3) Rabbit mAb #2128 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

Confocal immunofluorescent analysis of HeLa cells using CENP-A Antibody #2186 (green). Actin filaments have been labeled with Alexa Fluor^® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

IF-IC

Confocal immunofluorescent analysis of Caki-1 cells using NUP98 (C39A3) Rabbit mAb #2598 (green). Actin filaments have been labeled with Alexa Fluor^® 555 phalloidin (red).

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Fibrillarin (C13C3) Rabbit mAb #2639 (green). Actin filaments have been labeled with Alexa Fluor^® 555 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Calnexin (C5C9) Rabbit mAb #2679 (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Rab5 (C8B1) Rabbit mAb #3547 (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or chloroquine-treated (right), using LC3B (D11) XP^® Rabbit mAb #3868 (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Histone H3 (D1H2) XP^® Rabbit mAb #4499 (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor^® 555 Conjugate) #2116 (red).

IF-IC

Confocal immunofluorescent analysis of HeLa cells labeled with COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ™ (fluorescent DNA dye).

バックグラウンド

Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。

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本製品は試験研究用です。

Cellular Localization IF Antibody Sampler Kit