NF-kappaB Signaling

NF-κB p65 Antibody Sampler Kit

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希望納入価格 (円)
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2012年2月8日17時20分 現在
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#4767S1 Kit80,000
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NFkB-p65抗体製品一覧

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キット内容 容量 用途 種交差性 検出タンパク質の分子量 Isotype
Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 40 µl W IP IF-IC F H M R Hm Mk Pg (Dg) 65 Rabbit IgG
Acetyl-NF-κB p65 (Lys310) Antibody #3045 40 µl W IP H M (R) (Mk) (B) (Dg) 65 Rabbit
NF-κB p65 (L8F6) Mouse mAb #6956 40 µl W IP IHC-P IF-IC F ChIP H M R Hm Mk Mi B Dg Pg 65 Mouse IgG2b
NF-κB p65 (D14E12) XP® Rabbit mAb #8242 40 µl W IP IHC-P IF-IC F ChIP H M R Hm Mk Dg 65 Rabbit IgG
Phospho-NF-κB p65 (Ser468) Antibody #3039 40 ml W IP H M R 65 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl Horse
貯法
-20℃
※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TNF-α treated (#2169, 20 ng/ml for 5 minutes), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) or NF-κB p65 Antibody #3034 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells treated for 5 minutes with TNF-α #2169 (20 ng/ml), Calyculin A #9902 (50 nM), or both compounds, using Phospho-NF-kB p65 (Ser468) Antibody #3039 (top) or NF-kB p65 Antibody #3034 (bottom).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with NF-κB or NF-κB plus CBP, using Acetyl-NF-κB (Lys310) Antibody #3045. Western blots were competed with acetylated peptide (middle) or non-acetylated peptide (right).


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NF-κB p65 (L8F6) Mouse mAb #6956.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NF-κB p65 (D14E12) XP® Rabbit mAb #8242.

バックグラウンド

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).

RelA/p65 is a subunit of the NF-κB transcription complex, which plays a crucial role in inflammatory and immune responses. The complex is composed of various homodimeric and heterodimeric Rel family member combinations, the activity of which is modulated by post-translational modifications including phosphorylation and acetylation. p65 phosphorylation by PKA and/or MSK1 at Ser276 allows for increased interaction with the transcriptional coactivator p300/CBP to enhance transcriptional activity. NF-κB dimer assembly with IκB, as well as its DNA binding and transcriptional activities, are regulated by p300/CBP acetyltransferases that principally target Lys218, Lys221 and Lys310 (12-14). This process is reciprocally regulated by histone deacetylases (HDACs); several HDAC inhibitors have been shown to activate NF-κB (12-14). T-cell co-stimulation and Calyculin A have both been shown to increase Ser468 phosphorylation (15,16). IKKβ (but not IKKα) and GSK-3β both target this site (16,17), which appears to have a negative regulatory role not involving inhibition of nuclear translocation after TNF-α or IL-1β stimulation (17). p65 phosphorylation at Ser536 regulates activation, nuclear localization, protein-protein interactions, transcriptional activity, and apoptosis (18-22).

  1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
  2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
  3. Haskill, S. et al. (1991) Cell 65, 1281-9.
  4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
  5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
  6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
  7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
  8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
  9. Senftleben, U. et al. (2001) Science 293, 1495-9.
  10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
  11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.
  12. Ashburner, B.P. et al. (2001) Mol Cell Biol 21, 7065-77.
  13. Mayo, M.W. et al. (2003) J Biol Chem 278, 18980-9.
  14. Chen, L.F. et al. (2002) EMBO J 21, 6539-48.
  15. Mattioli, I. et al. (2004) Blood 104, 3302-4.
  16. Buss, H. et al. (2004) J Biol Chem 279, 49571-4.
  17. Schwabe, R.F. and Sakurai, H. (2005) FASEB J 19, 1758-60.
  18. Doyle, S.L. et al. (2005) J Biol Chem 280, 23496-501.
  19. Sasaki, C.Y. et al. (2005) J Biol Chem 280, 34538-47.
  20. Mattioli, I. et al. (2004) J Immunol 172, 6336-44.
  21. Bae, J.S. et al. (2003) Biochem Biophys Res Commun 305, 1094-8.
  22. Buss, H. et al. (2004) J Biol Chem 279, 55633-43.
使用例
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4727   c-Rel Antibody
4766   NF-κB Family Member Antibody Sampler Kit
4806   Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb
4808   Phospho-NF-κB p105 (Ser933) (178F3) Rabbit mAb (IHC Specific)
4810   Phospho-NF-κB2 p100 (Ser866/870) Antibody
4882   NF-κB2 p100/p52 Antibody
4922   RelB (C1E4) Rabbit mAb
7071   Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
9243   NF-κB Control Cell Extracts
9936   NF-κB Pathway Sampler Kit

本製品は試験研究用です。

NF-κB p65 Antibody Sampler Kit

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