NF-kappaB Signaling
Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb
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| #4806S | 100 μL | 57,000 | |
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NFkB-p105抗体製品一覧
4806 の推奨プロトコール
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推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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4806:
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Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:100） |
| 特異性・感度 | |
| 内在性レベルのSer933 がリン酸化されたNF-κB p105 タンパク質を検出します。 |
| 使用抗原 | |
| NF-κB p105 タンパク質のSer933 周辺のアミノ酸配列（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from Vero cells, untreated or treated with TNF-α #2169 (20 ng/ml) for the times indicated, using Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806 (upper) and NF-κB p105/p50 Antibody #3035 (lower).
Western Blotting
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated with TNF-α (#2169, 20 ng/ml) plus Calyculin A (#9902, 50 nM) for 5 minutes, using Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb.
Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
Following IKK-mediated phosphorylation of p105 NF-κB at multiple sites (Ser921, 923, 927, and 933) on its carboxy-terminus, SCFbeta-TrCP mediated processing produces the 50kDa active form p50 (12,13).
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Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
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Orian, A. et al. (2000) EMBO J. 19, 2580-2591.
PhosphoSitePlus®：
NFkB-p105