Translational Control
|
| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年3月11日15時15分 現在 |
|
|---|---|---|---|---|
| #4858S | 100 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
| |
| #4858L | 300 μL | 137,000 |
|
推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
|---|
| 用途（希釈倍率） | |
|---|---|
| ウェスタンブロッティング（1:2,000）、免疫組織染色（パラフィン）（1:50）、免疫組織染色（凍結）（1:50）、免疫蛍光細胞染色（IF-IC）（1:100）、フローサイトメトリー（1:25） | |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、出芽酵母 | |
| 特異性・感度 | |
|---|---|
| 内在性レベルのSer235 とSer236 がリン酸化されたS6 Ribosomal Protein を検出します。 | |
| 検出タンパク質の分子量 | |
|---|---|
| 32 kDa | |
| 使用抗原 | |
|---|---|
| ヒトのS6 Ribosomal Protein のSer235/236 周辺領域（合成リン酸化ペプチド） | |
| 抗体の由来 | |
|---|---|
| ウサギ | |
| 貯法 | |
|---|---|
| -20℃ | |
| 社内データ |
|---|
Western Blotting
Western blot analysis of extracts from PC12 and NIH/3T3 cells, treated with λ phosphatase, 20% FBS (20 min) or 100 ng/ml PDGF (20 min) as indicated, using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb in the presence of control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse spleen using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded A549 xenograft, untreated (left) or λ phosphatase-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb.
IHC-F (frozen)
Immunohistochemical analysis of frozen U-87MG xenograft using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, wortmannin and U0126 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (left) or 20% serum-treated (right), using Phospho-S6 Ribosomal protein (Ser235/Ser236) (D57.2.2E) XP™ Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
| バックグラウンド |
|---|
One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240 and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
- Dufner, A. and Thomas, G. (1999) Exp. Cell Res. 253, 100-109.
- Peterson, R.T. and Schreiber, S.L. (1998) Curr. Biol. 8, R248-R250.
- Jefferies, H.B. et al. (1997) EMBO J. 16, 3693-3704.
- Ferrari, S. et al. (1991) J. Biol. Chem. 266, 22770-22775.
- Flotow, H. and Thomas, G. (1992) J. Biol. Chem. 267, 3074-3078.
| 使用文献 | |
|---|---|
|
本製品は試験研究用です。