NF-kappaB Signaling
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| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年9月9日14時20分 現在 |
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| #4964S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途（希釈倍率） | |
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| ウェスタンブロッティング（1:1,000）、免疫沈降（1:100）、免疫蛍光細胞染色（IF-IC）（1:400）、フローサイトメトリー（1:400）、ChIP（1:25） | |
| 種交差性 | |
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| ヒト | |
| 特異性・感度 | |
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| 内在性レベルのIRF-4 タンパク質を検出します。生理的レベルの他のファミリータンパク質とは交差しません。 | |
| 検出タンパク質の分子量 | |
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| 51 kDa | |
| 使用抗原 | |
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| ヒトのIRF-4 タンパク質のAsp175 周辺領域（合成ペプチド） | |
| 抗体の由来 | |
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| ウサギ | |
| 貯法 | |
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| -20℃ | |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from Karpas-620 (human) and A20 (mouse) cell lines using IRF-4 Antibody #4964 and IRF-4 (P173) Antibody #4948.
Western Blotting
Western blot analysis of extracts from Ramos, Raji, and HuT-78 cells, using IRF-4 Antibody.
Flow Cytometry
Flow cytometric analysis of THP-1 cells (blue) and RPMI8226 cells (green) using IRF-4 Antibody.
IF-IC
Confocal immunofluorescent analysis of RPMI-8226 (left) and HeLa cells (right) using IRF-4 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 H929 cells and either 20 μl of IRF-4 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP™ Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP™ Human SUB1 Promoter Primers #5156 and SimpleChIP™ Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
| バックグラウンド |
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Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7 and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2).
IRF-4 was independently cloned by three groups and demonstrated to have roles in different contexts of lymphoid regulation (3-5). First, IRF-4 (Pip) was found to associate with PU.1, a hematopoietic specific member of the ETS family, and to regulate the expression of B-cell specific genes (3). Second, it was characterized as a lymphoid-specific member of the IRF family (LSIRF) and able to bind to ISRE (4). Third, it was identified in activated T cells as a factor that binds to the promoter of the interleukin-5 gene (ICSAT), and shown to repress gene activation induced by IFN (5). IRF-4 is expressed in all stages of B cell development and in mature T cells, and is inducible in primary lymphocytes by antigen mimetic stimuli such as Concavalin A, CD3 crosslinking, anti-IgM and PMA treatment (4,5). Mice deficient in IRF-4 show normal distribution of B and T lymphocytes at 4 to 5 weeks, but later develop progressive generalized lymphadenopathy, suggesting a role for IRF-4 in the function and homeostasis of mature B- and T-lymphocytes (6).
- Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
- Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
- Eisenbeis, C.F. et al. (1995) Genes Dev 9, 1377-87.
- Matsuyama, T. et al. (1995) Nucleic Acids Res 23, 2127-36.
- Yamagata, T. et al. (1996) Mol Cell Biol 16, 1283-94.
- Mittrücker, H.W. et al. (1997) Science 275, 540-3.
| 使用文献 |
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- Martin, H.J. et al. (2007) J Virol 81, 9748-58. Applications: Western Blotting
本製品は試験研究用です。
