Wnt / Hedgehog / Notch
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #5232S | 100 μL | 46,000 | |
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NANOG抗体製品一覧
5232 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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5232:
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ChIP Agarose
ChIP Magnetic
| 特異性・感度 | |
| 内在性レベルのNanog タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのNanog タンパク質のN末端周辺領域（合成ペプチド） |
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NTERA-2 cells and 10 μl of Nanog, Oct-4 and Sox2 antibodies, or 2 μl of Normal Rabbit IgG, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human Nanog promoter primers, SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 μl of Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and Lin28 (2,3).
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Kim, J. et al. (2008) Cell 132, 1049-61.
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Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
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Yu, J. et al. (2007) Science 318, 1917-20.
PhosphoSitePlus®：
