Nuclear Receptor Signaling
| CSTコード |
包装 |
希望納入価格 (円) |
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| #5388S | 100 μL | 46,000 | |
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RXRalpha抗体製品一覧
5388 の推奨プロトコール
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推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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5388:
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Immunoprecipitation
Western Blotting
| 用途(希釈倍率) | |
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50) |
| 特異性・感度 | |
| 内在性レベルのRXRα タンパク質を検出します。RXRβあるいはRXRγタンパク質とは交差しません。 |
| 使用抗原 | |
| ヒトのRXRαタンパク質のN末端近傍領域 (合成ペプチド) |
Western Blotting
Western blot analysis of extracts from various cell lines using RXRα Antibody.
Western Blotting
Western blot analysis of extracts from 293T cells, mock transfected or transfected with human RXRα, RXRβ, RXRγ DYKDDDDK tagged constructs, using RXRα Antibody (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (lower).
The human retinoid X receptors (RXRs) are encoded by three distinct genes (RXRα, RXRβ, and RXRγ) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid. RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor (1). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to cis-acting response elements in the promoter/enhancer region of target genes (2).
Retinoid X receptor α (RXRα) is the founding RXR family member and is predominantly expressed in liver, kidney, epidermis, intestine and a variety of tissues (2-4). Knockout of the murine rxrα gene results in embryonic lethality tentatively due to myocardial hypoplasia, which demonstrates the importance of RXRα to retinoid signaling in vivo (5,6). Biochemical evidence suggests that RXRα transcriptional activity is post-translationally regulated through the Ras-Raf-MAPK signaling cascade. MAPK-dependent phosphorylation of RXRα directly abrogates the ability of RXRα to associate with nuclear receptor coactivators (7).
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Gronemeyer, H. et al. (2004) Nat Rev Drug Discov 3, 950-64.
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Mangelsdorf, D.J. et al. (1992) Genes Dev 6, 329-44.
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Mangelsdorf, D.J. et al. (1990) Nature 345, 224-9.
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Dollé, P. et al. (1994) Mech Dev 45, 91-104.
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Kastner, P. et al. (1994) Cell 78, 987-1003.
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Sucov, H.M. et al. (1994) Genes Dev 8, 1007-18.
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Macoritto, M. et al. (2008) J Biol Chem 283, 4943-56.
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