MAP Kinase Signaling
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #5464S | 100 μL | 61,000 | |
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JUN抗体製品一覧
5464 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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5464:
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Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000） |
| 特異性・感度 | |
| 内在性レベルのSer63 がリン酸化されたc-Jun タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのc-Jun タンパク質のSer63 周辺領域（合成リン酸化ペプチド） |
Western Blotting
Western blot analysis of extracts from C6 cells, untreated or anisomycin-treated, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb (Biotinylated) and detected with Streptavidin-HRP #3999.
c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).
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Jochum, W. et al. (2001) Oncogene 20, 2401-12.
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Davis, R.J. (2000) Cell 103, 239-52.
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Hilberg, F. et al. (1993) Nature 365, 179-81.
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Raivich, G. et al. (2004) Neuron 43, 57-67.
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Behrens, A. et al. (2002) EMBO J 21, 1782-90.
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Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.
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Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
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Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
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Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
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Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.
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Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.
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Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.
PhosphoSitePlus®：
