Apoptosis and Autophagy
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
ご登録代理店情報 カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更は こちら。 |
| #6212S | 300 μL | 50,000 | |
|
LC3B抗体製品一覧
| 特異性 | |
| LC3B タンパク質の発現を特異的に抑制します。 |
Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence
®
Control siRNA (Unconjugated) #6568 (-), SignalSilence
®
LC3B siRNA I (+) or SignalSilence
®
LC3B siRNA II #6213 (+), using LC3B (D11) XP™ Rabbit mAb #3868 and α-Tubulin (11H10) Rabbit mAb #2125. The LC3B (D11) XP™ Rabbit mAb confirms silencing of LC3B expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of LC3B siRNA.
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form LC3-II have been used as indicators of autophagy (11).
-
Reggiori, F. and Klionsky, D.J. (2002)
Eukaryot. Cell
1, 11-21.
-
Codogno, P. and Meijer, A.J. (2005)
Cell Death Differ.
12 Suppl 2, 1509-1518.
-
Levine, B. and Yuan, J. (2005)
J. Clin. Invest.
115, 2679-2688.
-
Mann, S.S. and Hammarback, J.A. (1994)
J. Biol. Chem.
269, 11492-11497.
-
Lang, T. et al. (1998)
EMBO J.
17, 3597-3607.
-
Kabeya, Y. et al. (2000)
EMBO J.
19, 5720-5728.
-
He, H. et al. (2003)
J. Biol. Chem.
278, 29278-29287.
-
Tanida, I. et al. (2004)
J. Biol. Chem.
279, 47704-47710.
-
Wu, J. et al. (2006)
Biochem. Biophys. Res. Commun.
339, 437-442.
-
Ichimura, Y. et al. (2000)
Nature
408, 488-492.
-
Kabeya, Y. et al. (2004)
J. Cell Sci.
117, 2805-2812.
