MAP Kinase Signaling
- MAP Kinase Signaling
PhosphoSitePlus®：
HSP27
SignalSilence® HSP27 siRNA I |
イイネ！(0) | 
|
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
|
|---|---|---|---|---|
| #6356S | 300 μL | 50,000 | ログインすると国内在庫状況がご確認いただけます。
|
| テストした細胞種 | |
|---|---|
| HeLa（ヒト） |
| 特異性 | |
|---|---|
| HSP27 タンパク質の発現を抑制します。 |
| 貯法 | |
|---|---|
| -20℃ |
| 社内データ |
|---|
Western Blotting
Western blot analysis of extracts from HeLa cells transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or HSP27 siRNA I (+), using HSP27 (G31) Mouse mAb #2402 and p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107. The HSP27 (G31) mAb confirms silencing of HSP27 expression and p44/42 MAPK mAb is used to control for loading and specificity of HSP27 siRNA.
Fluorescent Detection
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.
| バックグラウンド |
|---|
Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).
Small interfering RNA (siRNA) has been used to specifically silence HSP27 expression in HeLa cells (7).
- Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.
- Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.
- Rouse, J. et al. (1994) Cell 78, 1027-1037.
- Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.
- Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.
- Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.
- Park, K. et al. (2003) J. Biol. Chem. 278, 35272-35278.
| 使用例 | |
|---|---|
| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。