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| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年7月30日15時15分 現在 |
|
|---|---|---|---|---|
| #7003 | 1 Pack | 32,000 | ログインすると国内在庫状況がご確認いただけます。
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| 内容 | |
|---|---|
| 20X LumiGLO® (luminol chemiluminescent substrate) 20X Peroxide | |
| 容量 | |
|---|---|
| 25 mL 25 mL | |
| 製品概要 | |
|---|---|
| HRP 標識2次抗体を用いたウエスタンブロッティングの検出に用いる試薬です。非常に高感度に検出することが可能です。 | |
| 貯法 | |
|---|---|
| 4℃ | |
| LumiGLO®はKirkegaard & Perry Laboratoriesの登録商標です。 |
| 社内データ |
|---|
Western Blotting
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO ® is added and emits light during enzyme catalyzed decomposition.
| バックグラウンド |
|---|
Chemiluminescent detection systems have emerged as the best all-around method for detection of western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. Horseradish peroxidase (HRP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. Horseradish peroxidase-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.
| 使用文献 |
|---|
- Kajiya, M. et al. (2008) J Biol Chem 283, 16259-67. Applications: Western Blotting
本製品は試験研究用です。
