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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月3日18時20分 現在 |
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|---|---|---|---|---|
| #7003S | 1 Pack | 32,000 | ログインすると国内在庫状況がご確認いただけます。
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| 内容 | 容量 | |
|---|---|---|
| 20X LumiGLO® (luminol chemiluminescent substrate) 20X Peroxide | 25 mL 25 mL |
| 製品概要 | |
|---|---|
| HRP 標識二次抗体を用いたウェスタンブロッティングの検出に用いる試薬です。非常に高感度に検出することが可能です。 |
| 貯法 | |
|---|---|
| 4℃ |
| LumiGLO®はKirkegaard & Perry Laboratories の登録商標です。 |
| 社内データ |
|---|
Western Blotting
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
| バックグラウンド |
|---|
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
| 使用文献 |
|---|
- Kajiya, M. et al. (2008) J Biol Chem 283, 16259-67. Applications: Western Blotting
本製品は試験研究用です。