Related Products
Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #7071S | 1 Kit (50回) | 53,000 | |
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| Kit 情報 | |
| 発光基質にLumiGLO Reagent を用いたペルオキシダーゼ（HRP）系ウエスタンブロット用化学発光検出キットです。 |
| キット内容 |
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| 製品 | 容量 | 抗体の由来 |
| Anti-rabbit IgG, HRP-linked Antibody | 50 ブロッティング用 | ヤギ |
| Anti-biotin, HRP-linked Antibody | 50 ブロッティング用 | ヤギ |
| Biotinylated Protein Ladder Detection Pack | 50 ブロッティング用 | |
| 20X LumiGLO® Reagent and 20X Peroxide | 50 ブロッティング用 | |
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本キットは長期保存の際の保管温度が異なる試薬が含まれています。製品受領後は製品のラベルの記載に従って保管してください。
LumiGLO®はKirkegaard & Perry Laboratoriesの登録商標です。
MSDSはこちらから |
Western Blotting
Western Blotting
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
