Related Products
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| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年7月30日15時15分 現在 |
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| #7072 | 50 mini-blots | 53,000 | ログインすると国内在庫状況がご確認いただけます。
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| Kit 情報 | |
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| 発光基質にLumiGLO Reagent を用いたペルオキシダーゼ（HRP）系ウエスタンブロット用化学発光検出キットです。 | |
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| 貯法 | |
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| -20℃ | |
| 本キットは長期保存の際の保管温度が異なる試薬が含まれています。製品受領後は製品のラベルの記載に従って保管してください。 LumiGLO®はKirkegaard & Perry Laboratoriesの登録商標です。 MSDSはこちらから |
| 社内データ |
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Western Blotting
Western Blotting
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO ® is added and emits light during enzyme catalyzed decomposition.
| バックグラウンド |
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Chemiluminescent detection systems have emerged as the best all-around method for detection of western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. Horseradish peroxidase (HRP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. Horseradish peroxidase-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
| 関連製品 | |
|---|---|
| 7003 20X LumiGLO Reagent and 20X Peroxide | |
| 7076 Anti-mouse IgG, HRP-linked Antibody | |
| 7720 Prestained Protein Marker, Broad Range (Premixed Format) | |
| 7727 Biotinylated Protein Ladder Detection Pack |
本製品は試験研究用です。
