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Anti-mouse IgG, HRP-linked Antibody |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
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|---|---|---|---|---|
| #7076S | 1 mL | 27,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000-3,000) |
| 抗体の由来 | |
|---|---|
| ウマ |
| 貯法 | |
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| -20℃ |
| 社内データ |
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Western Blotting
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.
| バックグラウンド |
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Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
| 使用文献 |
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- Wu, M. et al. (2010) Rejuvenation Res 13, 571-9. Applications: Western Blotting
本製品は試験研究用です。