Products
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月7日15時25分 現在 |
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| #7100S | 1 Kit | 96,000 | ログインすると国内在庫状況がご確認いただけます。
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7100 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 5301:
- Western Blotting
| Kit 情報 | |
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| 抗体の除去および抗体の再反応を行わずに、一枚のメンブレン上で数種類のシグナル伝達の活性化を分析することができるユニークなキットです。 |
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| 貯法 | |
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| -20℃ |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 MSDSはこちらから |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from CHO cells, untreated or insulin-treated following pretreatment with wortmannin (PI3 kinase inhibitor), rapamycin (mTOR inhibitor) and/or U0126 (MEK inhibitor) as indicated, using PathScan® Multiplex Western Cocktail I to detect phosphorylation of p90RSK, Akt, p44/42 MAPK and S6 ribosomal protein.
| バックグラウンド |
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Akt, also referred to as PKB or Rac, plays a critical role in controlling the balance between survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors, and functions in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and also by phosphorylation within the carboxy-terminus at Ser473.Both p44 and p42 MAP kinases (Erk1 and Erk2) play a critical role in the regulation of cell growth and differentiation (5-8). MAP kinases are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK) (9,10). One of the downstream targets of p44/42 MAPK is p90RSK.To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (11,12). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5+ untranslated regions (12).
- Franke, T.F. (1997) Cell 88, 435-437.
- Burgering, B.T. and Coffer, P.J. (1995) Nature 376, 599-602.
- Franke, T. F. et al. (1995) Cell 81, 727-736.
- Alessi, D.R. et al. (1996) EMBO J. 15, 6541-6551.
- Marshall, C.J. (1995) Cell 80, 179-185.
- Hunter, T. (1995) Cell 80, 225-236.
- Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211.
- Cowley, S. et al. (1994) Cell 77, 841-852.
- Sturgill, T.W. et al. (1988) Nature 334, 715-718.
- Payne, D. M. et al. (1991) EMBO J. 10, 885-892.
- Dufner, A. and Thomas, G. (1999) Exp. Cell. Res. 253, 100-109.
- Peterson, R.T. and Schreiber, S.L. (1998) Curr. Biol. 8, R248-R250.
| 使用文献 |
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- Steinbach, J. P. et al. (2004) Inhibition of Epidermal Growth Factor Receptor Signaling Protects Human Malignant Glioma Cells from Hypoxia-Induced Cell Death. Cancer Res. 64, 1575-1578. Applications: Western Blotting
- Yue, Y. et al. (2004) Ras GTPase-activating protein binds to Akt and is required for its activation. J. Biol. Chem. 279, 12883-12889. Applications: Western Blotting
本製品は試験研究用です。