PI3K / Akt Signaling
PathScan® RTK Signaling Antibody Array Kit (Fluorescent Readout) |
イイネ！(0) | 
|
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
|
|---|---|---|---|---|
| #7949S | 1 Kit | 135,000 | ログインすると国内在庫状況がご確認いただけます。
|
| キット内容 | 容量 | キャップの色 |
|---|---|---|
| Array Slides | 2 | |
| Multi-Well Gasket | 2 | |
| Sealing Tape | 2 sheets | |
| Detection Antibody Cocktail (10X ) | 300 µl | White |
| DyLight 680®-linked Streptavidin (10X) | 300 µl | Brown |
| Array Blocking Buffer | 5 ml | Red |
| Array Diluent Buffer | 15 ml | Blue |
| 20X Array Wash Buffer | 15 ml | White |
| Cell Lysis Buffer (10X) #9803 | 15 ml | Clear |
Note: Kit should be stored at 4°C with the exception of Cell Lysis Buffer (10X), which is stored at –20°C (packaged separately).
| Kit 情報 | |
|---|---|
| サンドイッチ免疫アッセイに基づいて作製されたスライドベースの抗体アレイです。チロシンあるいは他の残基がリン酸化された28 の受容体型チロシンキナーゼ（RTK）と11 の重要なシグナリング分子の同時検出が可能です。 |
| 推奨タンパク濃度 | |
|---|---|
| 0.2-1 mg/mL |
| 種交差性 | |
|---|---|
| ヒト |
| 貯法 | |
|---|---|
| 4℃（10X Cell Lysis Bufferは-20℃） |
| ※Lysis Buffer は保存温度が-20℃となっておりますが、輸送時においては保冷（冷媒梱包）とさせて頂いています。本品受領後は速やかに冷凍庫（-20℃）への保存をお願いいたします。 MSDSはこちらから |
| 社内データ |
|---|
Figure 1. Screening of cell lines using the PathScan® RTK Signaling Antibody Array (Fluorescent Readout) #7949 reveals various phosphorylated RTKs and signaling nodes. Karpas-299 and K562 cells were lysed without starvation or treatment. The fluorescent image (lower panel) and the quantification of that image (upper panel) are shown.
Figure 2. Treatment of MCF-7 cells with IGF-I stimulates phosphorylation of IGF-IR at tyrosine residues, Akt at Ser473 and p44/42 MAPK at Thr202/Tyr204 as detected by the PathScan® RTK Signaling Antibody Array Kit (Fluorescent Readout) #7949. MCF-7 cells were starved for 24 hours, then treated with 100 ng/ml IGF-I #3093 for 5 minutes at 37ºC. The fluorescent image (lower panel) and the quantification of that image (upper panel) are shown.
Figure 3. Treatment of A431 cells with EGF stimulates phosphorylation of EGFR, Akt, p44/42 MAPK and Stat3 as detected by the PathScan® RTK Signaling Antibody Array Kit (Fluorescent Readout) #7949. A431 cells were starved for 24 hours and treated with 100 ng/ml hEGF #8916 for 5 or 40 minutes. In some cases, cells were treated with either 1 μM wortmannin #9951 for 1 hour or 1 μM gefitinib for 2 hours before EGF stimulation. Fluorescence intensities obtained from the array are shown in the top panel, while western blots are shown in the bottom panel.
Figure 4. The relationship between lysate protein concentration from untreated and IGF-I treated MCF-7 cells and the relative fluorescence of phospho-IGF-IR (panTyr), phospho-Akt (Ser473) and phospho-p44/42 (Thr202/Tyr204) is shown. MCF-7 cells were starved for 24 hours, then treated with 100 ng/ml IGF-I #3093 for 5 minutes at 37ºC.
| バックグラウンド |
|---|
Receptor Tyrosine Kinases (RTKs) are a family of cell surface receptors that signal primarily through tyrosine phosphorylation events (1). RTKs trigger a wide range of downstream signaling cascades, including the PI3K/Akt, MAPK and Jak/Stat pathways. These pathways control basic cellular functions such as division, growth, metabolism, differentiation, migration and survival. Dysregulation of RTK signaling has been implicated in a large number of cancers (2), making RTKs popular targets for pharmaceutical intervention.
| 使用例 | |
|---|---|
| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
| 関連製品 | |
|---|---|
| 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100) | |
| 9803 Cell Lysis Buffer (10X) |
本製品は試験研究用です。