MAP Kinase Signaling
PathScan® RTK Signaling Antibody Array Kit (Chemiluminescent Readout) |
イイネ！(9) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #7982S | 1 Kit | 126,000 | ログインすると国内在庫状況がご確認いただけます。
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| キット内容 | 容量 | キャップの色 |
|---|---|---|
| Array Slides | 2 | |
| Multi-Well Gasket | 2 | |
| Sealing Tape | 2 sheets | |
| Chemiluminescent Development Folder | 2 | |
| Detection Antibody Cocktail (10X ) | 300 µl | White |
| HRP-Linked Streptavidin (10X) | 300 µl | Clear |
| Array Blocking Buffer | 5 ml | Red |
| Array Diluent Buffer | 15 ml | Blue |
| 20X Array Wash Buffer | 15 ml | White |
| 20X LumiGLO Reagent A | 5 ml | Brown |
| 20X Peroxide Reagent B | 5 ml | Clear |
| Cell Lysis Buffer (10X) #9803 | 15 ml | Clear |
Note: Kit should be stored at 4°C with the exception of Cell Lysis Buffer (10X), which is stored at –20°C (packaged separately).
| Kit 情報 | |
|---|---|
| サンドイッチ免疫アッセイに基づいて作製されたスライドベースの抗体アレイです。チロシンあるいは他の残基がリン酸化された28 の受容体型チロシンキナーゼ（RTK）と11 の重要なシグナリング分子の同時検出が可能です。 |
| 推奨タンパク濃度 | |
|---|---|
| 0.2-1 mg/mL |
| 種交差性 | |
|---|---|
| ヒト |
| 貯法 | |
|---|---|
| 4℃（10X Cell Lysis Bufferは-20℃） |
| ※本品は保存温度が-20℃となっておりますが、輸送時においては保冷（冷媒梱包）とさせて頂いています。本品受領後は速やかに冷凍庫（-20℃）への保存をお願いいたします。 MSDSはこちらから |
| 社内データ |
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Figure 1. Screening of a panel of cell lines using the PathScan® RTK Signaling Antibody Array Kit (Chemiluminescent Readout) #7982 reveals various phosphorylated RTKs and signaling nodes. A431 cells were starved for 24 hours, then treated with 100 ng/ml EGF #8916 for 3 minutes at 37ºC. NIH/3T3 cells were starved for 24 hours, then treated with 100 ng/ml PDGF #9909 for 5 minutes at 37ºC. Karpas-299 and K562 cells were lysed without starvation or treatment. The array images were captured using chemiluminescent film, with 2-5 second exposure times.
Figure 2. Treatment of MCF-7 cells with IGF-I stimulates phosphorylation of IGF-IR at tyrosine residues, Akt at Ser473 and p44/42 MAPK at Thr202/Tyr204 as detected by the PathScan® RTK Signaling Antibody Array Kit (Chemiluminescent Readout) #7982. MCF-7 cells were starved for 24 hours, then treated with 100 ng/ml IGF-I #3093 for 5 minutes at 37ºC. The chemiluminescent film image (lower panel) and the quantification of that image (upper panel) are shown. The chemiluminescent array images were captured following 2-5 second film exposures.
Figure 3. Treatment of A431 cells with EGF stimulates phosphorylation of EGFR, Akt, p44/42 MAPK and Stat3 as detected by the PathScan® RTK Signaling Antibody Array Kit (Chemiluminescent Readout) #7982. A431 cells were starved for 24 hours and treated with 100 ng/ml EGF #8916 for 5 or 40 minutes. In some cases, cells were treated with either 1 μM wortmannin #9951 for 1 hour before or 1 μM gefitinib for 2 hours before EGF stimulation. Array image pixel intensities obtained from a digital imager are shown in the top panel, while western blots are shown in the bottom panel.
Figure 4. The relationship between lysate protein concentration from untreated and IGF-I treated MCF-7 cells and the pixel intensities of phospho-IGF-IR (pan-Tyr), phospho-Akt (Ser473) and phospho-p44/42 (Thr202/Tyr204) is shown. MCF-7 cells were starved for 24 hours, then treated with 100 ng/ml IGF-I #3093 for 5 minutes at 37ºC.
| バックグラウンド |
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Receptor Tyrosine Kinases (RTKs) are a family of cell surface receptors that signal primarily through tyrosine phosphorylation events (1). RTKs trigger a wide range of downstream signaling cascades, including the PI3K/Akt, MAPK and Jak/Stat pathways. These pathways control basic cellular functions such as division, growth, metabolism, differentiation, migration and survival. Dysregulation of RTK signaling has been implicated in a large number of cancers (2), making RTKs popular targets for pharmaceutical intervention.
| 使用例 | |
|---|---|
| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
| 関連製品 | |
|---|---|
| 7003 20X LumiGLO® Reagent and 20X Peroxide | |
| 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100) | |
| 9803 Cell Lysis Buffer (10X) |
本製品は試験研究用です。