Translational Control
SignalSlide® Phospho-Akt (Ser473) IHC Controls |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
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| #8101S | 1 Pack (5 Slides) | 26,000 | ログインすると国内在庫状況がご確認いただけます。
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- 8101:
- IHC / Paraffin
| 用途 | |
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| 免疫組織染色 |
| 製品概要 | |
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| Ser473 がリン酸化されたAkt タンパク質の免疫染色を確認するためのホルマリン固定/パラフィン包埋したPI3-Kinase 阻害剤のLY294002 未処理と処理済のLNCaP 細胞 |
| 貯法 | |
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| 4℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from LNCaP cells, untreated or treated with LY294002, using Akt Antibody #9272 (upper) orPhospho-Akt (Ser 473) (193H121) Rabbit mAb #4058 (lower). This assay serves as a control for the efficacy of the LY94002 treatment.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded LNCaP cells untreated (left) or treated with LY294002 (right) using Phospho-Akt (Ser473) (736E11) Rabbit mAb (IHC Specific) #3787.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or treated with LY294002 (right) using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060.
Schematic
| バックグラウンド |
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Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).
- Franke, T.F. et al. (1997) Cell 88, 435-7.
- Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
- Franke, T.F. et al. (1995) Cell 81, 727-36.
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- Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4.
- Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
- Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8.
- Hajduch, E. et al. (2001) FEBS Lett 492, 199-203.
- Cross, D.A. et al. (1995) Nature 378, 785-9.
- Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
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- Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52.
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- Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
| 使用例 | |
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本製品は試験研究用です。