Antibody Sampler Kits

SignalStain® Akt Pathway IHC Sampler Kit

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CSTコード 包装
希望納入価格 (円)
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2012年2月8日17時20分 現在
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#8107S1 Kit96,000
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Akt抗体製品一覧

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推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

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キット内容 容量 Antigen Retrieval / Diluent Isotype
Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060 40 µl Citrate / SignalStain®
Antibody Diluent #8112		 Rabbit IgG
Phospho-S6 Ribosomal Protein (Ser235/236)
(D57.2.2E) Rabbit mAb #4858		 40 µl Citrate / SignalStain®
Antibody Diluent #8112		 Rabbit IgG
Akt (pan) (C67E7) Rabbit mAb #4691 40 µl Citrate / SignalStain®
Antibody Diluent #8112		 Rabbit IgG
PTEN (D4.3) Rabbit mAb #9188 40 µl Citrate / SignalStain®
Antibody Diluent #8112		 Rabbit IgG
*SignalStain® Antibody Diluent #8112 25 ml
†SignalSlide™ Akt Family IHC Controls 1 Pack
貯法
-20℃（SignalStain® Antibody Diluent #8112 とSignalSlide™ Akt Family IHC Controls #8115 は4℃）
社内データ

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded LNCaP cell pellets, either untreated (left) or LY294002-treated (middle) or NIH/3T3 cell pellets (right), using Phospho-Akt (Ser473) (D9E) Rabbit mAb, Akt (pan) (C67E7) Rabbit mAb, PTEN (D4.3) Rabbit mAb and Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb. Cell pellets are provided in the SignalSlide™ Akt Family IHC Controls.

Schematic

Schematic

Schematic of placement of cell pellets on SignalSlide™ Akt Family IHC Controls #8115.

バックグラウンド

The protein kinase Akt (also called PKB or Rac) is activated by insulin and growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (1,2). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (3) and by carboxy terminal phosphorylation at Ser473 by the mTORC2 complex composed of mammalian target of rapamycin (mTOR) in a complex with rictor and Sin1 (4,5). Akt signaling is negatively regulated via the PTEN phosphatase (6).Akt’s several functions include inhibition of apoptosis (7-9), regulation of glycogen synthesis via GSK-3 (10), and promotion of the cell cycle (11). Akt also regulates protein synthesis by phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (mTORC1) (12). Akt also effects mTOR activity via phosphorylation and inhibition of PRAS40 (40 kDa, proline-rich protein), which binds to raptor in the mTORC1 complex and inhibits mTOR activity (13). Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (14), allowing protein synthesis to occur. Active of mTORC1 signals to p70 S6 kinase, which in turn phosphorylates S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of a subset of mRNA transcripts that encode ribosomal proteins, translation elongation factors as well as regulators of cell cycle progression (15). Important S6 ribosomal protein phosphorylation sites include Ser235, Ser236, Ser240 and Ser244 within a small carboxy-terminal region (16).

  1. Franke, T.F. et al. (1997) Cell 88, 435-7.
  2. Franke, T.F. et al. (1995) Cell 81, 727-36.
  3. Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
  4. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
  5. Jacinto, E. et al. (2006) Cell 127, 125-37.
  6. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
  7. Cardone, M.H. et al. (1998) Science 282, 1318-21.
  8. Brunet, A. et al. (1999) Cell 96, 857-68.
  9. Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4.
  10. Cross, D.A. et al. Nature 378, 785-9.
  11. Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52.
  12. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
  13. Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23.
  14. Sancak, Y. et al. (2007) Mol Cell 25, 903-15.
  15. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
  16. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
使用例
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関連製品
1085   Akt (pan) Blocking Peptide
1140   Phospho-Akt (Ser473) Blocking Peptide
1220   Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide
1250   PTEN Blocking Peptide
3900   Rabbit (DA1E) mAb IgG XP® Isotype Control
4060   Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb
4691   Akt (pan) (C67E7) Rabbit mAb
4858   Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb
8112   SignalStain® Antibody Diluent
9188   PTEN (D4.3) XP® Rabbit mAb

本製品は試験研究用です。

SignalStain® Akt Pathway IHC Sampler Kit

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