MAP Kinase Signaling
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
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| #8110S | 1 Kit | 96,000   | |
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p44/42 MAPK (Erk1/2)抗体製品一覧
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キット内容
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キャップの色 |
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Peroxidase Quench
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Orange
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Blocking Solution
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Blue
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Prediluted P-p44/42 MAPK (T202/Y204) (20G11) mAb #4376
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Purple
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Prediluted Negative Control
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Brown
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Biotinylated Secondary Ab
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Green
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Reagent A
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Gray
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Reagent B
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Gray
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NovaRED Substrate 1 (TM)
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Red
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NovaRED Substrate 2 (TM)
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Red
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NovaRED Substrate 3 (TM)
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Red
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NovaRED Substrate 4 (TM)
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Red
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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Blocking Peptide #
1150
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White
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| Kit 情報 | |
| 免疫組織染色を行うために必要な抗体や発色基質などが入ったキットです。 |
| 特異性・感度 | |
| 内在性レベルのThr202/Tyr204 がリン酸化されたp44 MAPK タンパク質（Erk1）を検出します。また、内在性レベルのThr185/Tyr187 がリン酸化されたp42 MAPK タンパク質（Erk2）も検出します。同様の領域がリン酸化されたSAPK/JNK あるいはp38 MAPK タンパク質とは交差しません。 |
NovaRED™ はVector Laboratories の登録商標です。
MSDSはこちらから |
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Growth/Proliferation Marker: SignalStain® Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Detection Kit (left). Serial section stained with matched negative control demonstrates specificity of staining (right).
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
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