Jak/Stat Pathway
SignalStain® Phospho-Stat IHC Sampler Kit |
イイネ！(1) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #8113S | 1 Kit | 85,000 | ログインすると国内在庫状況がご確認いただけます。
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* 受注生産につきお届けは受注後2-3週間かかります。キットに含まれるコントロールスライドの有効期限は製造後2ヶ月です。
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8113 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 8105:
- IHC / Paraffin
- 8112:
- IHC / Paraffin
- 9145:
- ChIP Agarose ChIP Magnetic Flow IHC / Frozen IHC / Paraffin Immunofluorescence* Immunoprecipitation Western Blotting
- 9167:
- ChIP Agarose ChIP Magnetic Flow IHC / Frozen IHC / Paraffin Immunofluorescence* Immunoprecipitation Western Blotting
- 9359:
- Flow IHC / Paraffin Immunoprecipitation Western Blotting
| キット内容 | 容量 | Antigen Retrieval / Diluent | Isotype |
|---|---|---|---|
| Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 | 40 µl | EDTA / SignalStain® Antibody Diluent #8112 | Rabbit IgG |
| Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb #9145 | 40 µl | EDTA / SignalStain® Antibody Diluent #8112 | Rabbit IgG |
| Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 | 40 µl | EDTA / SignalStain® Antibody Diluent #8112 | Rabbit IgG |
| *SignalStain® Antibody Diluent #8112 | 25 ml | ||
| †SignalSlide™ Phospho-Stat1/3/5 IHC Controls #8105 | 1 Pack |
| 貯法 | |
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| -20℃（SignalStain® Antibody Diluent #8112 とSignalSlide™ Phospho-Stat1/3/5 IHC Controls #8105 は4℃） |
| 社内データ |
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IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded 786-0 cell pellets, untreated (left) or IFN-α-treated (right) using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (upper), Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (middle) and Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb (lower). Cell pellets are provided in SignalSlide™ Phospho-Stat1/3/5 IHC Controls.
Schematic
| バックグラウンド |
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Stat proteins serve as transcription factors in growth and survival pathways stimulated by growth factor and cytokine activation of receptor proteins. Receptor activation promotes tyrosine phosphorylation of Stat proteins, resulting in Stat dimerization and translocation to the nucleus where they regulate expression of numerous proteins that control cell growth, survival, differentiation and pathogen resistance (1). Stat1 is essential in IFN-α and IFN-γ stimulated pathways and is abnormally activated in many tumors (2,3). Both Stat1α (91 kDa) and Stat1β (84 kDa) isoforms are activated by IFN-α but only Stat1α responds to IFN-γ. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Transcription factor Stat3 possesses oncogenic potential and anti-apoptotic activities; a number of human tumors display constitutively activated Stat3 (5,6). Activation of Stat3 follows phosphorylation at Tyr705, resulting in dimerization, nuclear translocation and DNA binding (7). Expression of Stat3α (86 kDa) and Stat3β (79 kDa) isoforms correlates with cell type, ligand and cell maturation stage (8). Phosphorylation of Stat5a at Tyr694 is essential for transcription factor activation (9). Stat5 is activated by interleukins and other cytokines (i.e. CSF1); presence of phosphorylated Stat5 is in some IL-3-treated cells suggests a role in angiogenesis and cell motility (10). Altered Stat5 expression and activity is associated with abnormal cell proliferation and apoptosis in some forms of leukemia (11).
- Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7.
- Bromberg, J. (2002) J Clin Invest 109, 1139-42.
- Frank, D.A. (1999) Mol Med 5, 432-56.
- Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
- Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
- Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
- Darnell, J.E. (1997) Science 277, 1630-5.
- Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
- Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.
- Buitenhuis, M. et al. (2004) Int J Biochem Cell Biol 36, 2120-4.
- Baśkiewicz-Masiuk, M. et al. (2003) Cell Prolif 36, 265-78.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。