Stem Cell Markers

StemLight™ iPS Cell Reprogramming Antibody Kit

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2012年2月7日15時25分 現在
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#9092S1 Kit91,000
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キット内容 容量 用途 種交差性 検出タンパク質の分子量 Isotype
Oct-4A (C30A3) Rabbit mAb #2840 40 µl W IF-IC F H M 45 Rabbit IgG
Sox2 (D6D9) XP® Rabbit mAb #3579 40 µl W IHC-P IF-IC F H (Mk) (B) (Dg) (Hr) 35 Rabbit IgG
Nanog (D73G4) XP® Rabbit mAb #4903 40 µl W IHC-P IF-IC F H (Mk) 42 Rabbit IgG
LIN28A (D84C11) XP® Rabbit mAb #3695 40 µl W IF-IC F H (R) (Mk) 26 Rabbit IgG
KLF4 Antibody #4038 40 µl W H M Mk 65 Rabbit
c-Myc (D84C12) XP® Rabbit mAb #5605 40 µl W IP IF-IC F H M R (Mk) (Dg) (Pg) 57-65 Rabbit IgG
貯法
-20℃
社内データ

IF-IC

IF-IC

Confocal immunofluorescent analysis of iPS cells using Oct-4A (C30A3) Rabbit mAb #2840, Sox2 (D6D9) XP® Rabbit mAb #3579, Nanog (D73G4) XP® Rabbit mAb #4903, c-Myc (D84C12) XP® Rabbit mAb #5605, and LIN28A (D84C11) XP® Rabbit mAb #3695 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of lentiviral transduced human fibroblast cells using Oct-4A (C30A3) Rabbit mAb #2840, Sox2 (D6D9) XP® Rabbit mAb #3579, c-Myc (D84C12) XP® Rabbit mAb #5605, and KLF4 Antibody #4038 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma and induced pluripotent cells.Oct-4, Sox2 and Nanog are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Over-expression of Oct-4 and Sox2 along with Klf4 and c- Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcrip- tional network necessary for renewal and pluripotency (4-5). It has also been demonstrated that overexpression of Oct-4, Sox2, Nanog and Lin28 can induce pluripotency in human somatic cells (6). Upon differentiation of pluripotent cultures, expression of Oct-4, Nanog and Sox2 is downregulated.SSEA4, TRA-1-81 and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (7). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (8). These epitopes are neurominadase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (9).

  1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
  2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  4. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
  5. Okita, K. et al. (2007) Nature 448, 313-7.
  6. Yu, J. et al. (2007) Science 318, 1917-20.
  7. Henderson, J.K. et al. (2002) Stem Cells 20, 329-37.
  8. Draper, J.S. et al. (2002) J Anat 200, 249-58.
  9. Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30.
使用例
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本製品は試験研究用です。

StemLight™ iPS Cell Reprogramming Antibody Kit

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