MAP Kinase Signaling
推奨アプリケーションで優れたパフォーマンスを発揮するXP®モノクローナル抗体をお試しください : 4370
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody |
イイネ！(19) |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
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| #9101S | 200 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9101L | 600 μL | 137,000 |
MAPK XP®モノクローナル抗体 | MAPK抗体製品一覧
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9101 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50)、免疫蛍光細胞染色 (IF-IC) (1:1,000)、フローサイトメトリー (1:200) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、ミンク、ブタ、ハムスター、ウシ、ゼブラフィッシュ、キイロショウジョウバエ、線虫、(ニワトリ) |
| 特異性・感度 | |
|---|---|
| 内在性レベルのThr202/Tyr204 両方あるいは片方だけがリン酸化されたp44 MAPK タンパク質 (Erk1) を検出します。また、内在性レベルのThr185/Tyr187 両方あるいは片方だけがリン酸化されたp42 MAPK タンパク質 (Erk2) も検出します。関連する残基がリン酸化されたSAPK/JNK あるいはp38 MAPK タンパク質とは交差しません。リン酸化していないp44/42 MAPK (Erk1/2) とも交差しません。 |
| 検出タンパク質の分子量 | |
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| 42 kDa、44 kDa |
| 使用抗原 | |
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| ヒトのp44 MAPK タンパク質のThr202/Tyr204 周辺領域 (合成リン酸化ペプチド) |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
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| -20℃ |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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推奨アプリケーションで優れたパフォーマンスを発揮するXP®モノクローナル抗体をお試しください : 4370
Western Blotting
Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase.
Western Blotting
Western blot analysis of whole-cell extracts from unstarved wild-type mouse embryonic fibroblasts (MEFs) treated with the indicated combinations of basic Fibroblast Growth Factor (bFGF #9952, 100 ng/ml for 30 minutes), Platelet-Derived Growth Factor (PDGF #9909, 100 ng/ml for 30 minutes), MEK1 Inhibitor (PD98059 #9900, 50 µM, 2 hour pre-treatment), and MEK1/2 Inhibitor (U0126 #9903, 10 µM, 2 hour pre-treatment), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101 (upper panel) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower panel).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or PMA-treated (blue), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody compared to a nonspecific negative control antibody (red).
Flow Cytometry
Phosphorylated MEK and Erk were assayed in human peripheral blood lymphocytes stimulated with PMA in the presence or absence of the Raf inhibitor BAY 37-9751 or the MEK inhibitor U0126 #9903. BAY 37-951 blocked PMA-stimulated phosphorylation of both MEK and Erk, consistent with inhibition at the level of Raf, while U0126 blocked phosphorylation of Erk only, consistent with inhibition at the level of MEK. From Chow, S. et al. (2001) Cytometry 46, 72-78.
IF-IC
Confocal immunofluorescent analysis of NIH/3T3 cells either U0126-treated (left) or PDGF-treated (right) and labeled with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
| バックグラウンド |
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Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
- Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
- Baccarini, M. (2005) FEBS Lett 579, 3271-7.
- Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
- Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
- Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
- Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Marais, R. et al. (1993) Cell 73, 381-93.
- Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
- Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
| 使用文献 |
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- Schulze, A. et al. (2001) Genes. Dev. 15, 981-94. Applications: Western Blotting
- Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4. Applications: Western Blotting
- Zhang, Y.W. et al. (2003) Proc. Natl Acad. Sci. USA 100, 12718-23. Applications: Western Blotting
- Patrucco, E. et al. (2004) Cell 118, 375-87. Applications: Western Blotting
- Yu, C. et al. (2002) Cancer Res. 62, 188-99. Applications: Western Blotting
- Dai, Y. et al. (2001) Cancer Res. 61, 5106-15. Applications: Western Blotting
- Chow, S. et al. (2001) Cytometry 46, 72-8. Applications: Flow Cytometry
- Kawagoe, T. et al. (2008) Nat Immunol 9, 684-91. Applications: Western Blotting
- Abe, T. et al. (2007) J Virol 81, 8953-66. Applications: Western Blotting
- Tsuda, M. et al. (2008) Glia 56, 378-86. Applications: IF-IC (In Cells)
- Moritz, A. et al. (2010) Sci Signal 3, ra64. Applications: Western Blotting
- Hosoi, T. et al. (2008) Mol Pharmacol 74, 1610-9. Applications: Western Blotting
- Kojima, R. et al. (2004) Biochem J 380, 783-94. Applications: Western Blotting
- Nakasaki, M. et al. (2008) Bone 43, 869-79. Applications: Western Blotting
- Oneyama, C. (2008) Mol Cell 30, 426-36. Applications: Western Blotting
- Kitamura, T. et al. (2008) Nat Cell Biol 10, 329-37. Applications: Western Blotting
- Nishimura, M. et al. (2009) Mol Cell Biol 29, 5529-39. Applications: Western Blotting
- Sakaue, H. et al. (2003) J Biol Chem 278, 38870-4. Applications: Western Blotting
本製品は試験研究用です。