MAP Kinase Signaling
p44/42 MAPK (Erk1/2) Antibody |
イイネ！(5) | 
|
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
|
|---|---|---|---|---|
| #9102S | 200 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
| |
| #9102L | 600 μL | 111,000 |
|
9102 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
|---|
下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50)、免疫組織染色 (パラフィン) (1:100)、免疫蛍光細胞染色 (IF-IC) (1:50)、フローサイトメトリー(1:50) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、ミンク、ブタ、ハムスター、ウシ、ゼブラフィッシュ、出芽酵母 |
| 特異性・感度 | |
|---|---|
| 内在性レベルのp44/42 MAPK (Erk1/Erk2) タンパク質を検出します。ある種の細胞ではp42 MAPK よりp44 MAPK に強く反応します。この抗体はJNK/SAPK やp38 MAPK は認識しません。 |
| 検出タンパク質の分子量 | |
|---|---|
| 42 kDa、44 kDa |
| 使用抗原 | |
|---|---|
| ラットのp44 MAPK のC末端の配列 (合成ペプチド) |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| 社内データ |
|---|
Western Blotting
Western blot analysis of extracts from HeLa cells transfected with 100 nM control siRNA #6201 (-) or p44 MAPK (Erk1) siRNA (+), using p44/42 MAPK (Erk1/2) Antibody #9102 and GCK Antibody #3782. The Erk1/2 antibody confirms silencing of Erk1 expression, and GCK Antibody is used to control for loading and specificity of p44 MAPK (Erk1) siRNA.
Western Blotting
Western blot analysis of extracts from serum-induced PC12 cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101 (upper) or control p44/42 MAPK (Erk1/2) Antibody (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p44/42 MAPK (Erk1/2) Antibody.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using p44/42 MAPK (Erk1/2) Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent images of C6 cells serum-starved (left) and serum-treated (center), labeled with p44/42 MAPK (Erk1/2) Antibody (green) compared to an isotype control (right). Actin filaments have been labeled with Alex Fluor® 555 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
| バックグラウンド |
|---|
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
- Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
- Baccarini, M. (2005) FEBS Lett 579, 3271-7.
- Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
- Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
- Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
- Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Marais, R. et al. (1993) Cell 73, 381-93.
- Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
- Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
| 使用文献 |
|---|
- Fei, Z. et al. (2011) J Biol Chem 286, 27761-8. Applications: Western Blotting
- Wu, M. et al. (2009) Diabetes Metab Res Rev 25, 279-86. Applications: Western Blotting
- Abe, T. et al. (2007) J Virol 81, 8953-66. Applications: Western Blotting
- Yu, C. et al. (2002) Cancer Res 62, 188-99. Applications: Western Blotting
- Rosenberger, C.M. and Finlay, B.B. (2002) J Biol Chem 277, 18753-62. Applications: Western Blotting
- Wu, J. et al. (2002) J Biol Chem 277, 19498-505. Applications: Western Blotting
- Domina, A.M. et al. (2000) J Biol Chem 275, 21688-94. Applications: Western Blotting
- Hayne, C. et al. (2000) J Biol Chem 275, 31876-82. Applications: Western Blotting
- Tan, J. et al. (2000) J Neurosci 20, 7587-94. Applications: Western Blotting
- Dai, Y. et al. (2001) Cancer Res 61, 5106-15. Applications: Western Blotting
- Kajiya, M. et al. (2008) J Biol Chem 283, 16259-67. Applications: Western Blotting
- Kojima, R. et al. (2004) Biochem J 380, 783-94. Applications: Western Blotting
- Nakasaki, M. et al. (2008) Bone 43, 869-79. Applications: Western Blotting
- Oneyama, C. (2008) Mol Cell 30, 426-36. Applications: Western Blotting
- Kitamura, T. et al. (2008) Nat Cell Biol 10, 329-37. Applications: Western Blotting
- Nishioka, Y. et al.(2008) Blood 111, 5086-92. Applications: Flow
本製品は試験研究用です。