MAP Kinase Signaling
推奨アプリケーションで優れたパフォーマンスを発揮するXP®モノクローナル抗体をお試しください : 4370
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb |
イイネ！(7) |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
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| #9106S | 200 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9106L | 600 μL | 137,000 |
MAPK XP®モノクローナル抗体 | MAPK抗体製品一覧
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9106 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:2,000)、免疫沈降 (1:50)、フローサイトメトリー (1:2,000) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、ミンク、ブタ、ハムスター、ウシ、ゼブラフィッシュ、キイロショウジョウバエ |
| 特異性・感度 | |
|---|---|
| 内在性レベルのThr202/Tyr204 両方あるいはTyr204 だけがリン酸化されたp44 MAPK タンパク質 (Erk1) を検出します。また、内在性レベルのThr185/Tyr187 両方がリン酸化されたp42 MAPKタンパク質 (Erk2) も検出します。関連する残基がリン酸化されたSAPK/JNK あるいはp38 MAPK タンパク質とは交差しません。免疫蛍光細胞染色による可視化で、いくつかの細胞株で未知の細胞骨格タンパク質と交差反応する可能性があります。 |
| 検出タンパク質の分子量 | |
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| 42 kDa、44 kDa |
| 使用抗原 | |
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| ヒトのp44 MAPK タンパク質のThr202/Tyr204 周辺領域 (合成リン酸化ペプチド) |
| 抗体の由来 | |
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| マウス |
| 貯法 | |
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| -20℃ |
| 社内データ |
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推奨アプリケーションで優れたパフォーマンスを発揮するXP®モノクローナル抗体をお試しください : 4370
Western Blotting
Specificity and sensitivity of the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb: This antibody reacts specifically with as little as 50 pg of phosphorylated MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated MAP kinase by Western blotting.
Western Blotting
Western blot analysis of extracts from FGF treated SK-N-MC cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (upper) or control p44/42 MAPK (Erk1/2) Antibody #9102 (lower).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (red) or PMA-treated (blue) ; and untreated (green) or PMA-treated (purple) following 90-minute CIP treatment, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb.
| バックグラウンド |
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Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
- Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
- Baccarini, M. (2005) FEBS Lett 579, 3271-7.
- Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
- Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
- Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
- Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Marais, R. et al. (1993) Cell 73, 381-93.
- Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
- Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
| 使用文献 |
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- Fonseca, B.D. et al. (2011) J Biol Chem 286, 27111-22. Applications: Western Blotting
- Fei, Z. et al. (2011) J Biol Chem 286, 27761-8. Applications: Western Blotting
- Xu, P. and Derynck, R. (2010) Mol Cell 37, 551-66. Applications: Western Blotting
- Feinerman, O. et al. (2008) Science 321, 1081-4. Applications: Flow Cytometry
- Hyduk, S.J. and Cybulsky, M.I. (2002) Alpha4 Integrin Signaling Activates Phosphatidylinositol 3-Kinase and Stimulates T Cell Adhesion to Intercellular Adhesion Molecule-1 to a Similar Extent As CD3, but Induces a Distinct Rearrangement of the Actin Cytoskeleton. The Journal of Immunology 168, 696-704. Applications: Western Blotting
- Zubiaur, M. et al. (2002) CD38 Is Associated with Lipid Rafts and upon Receptor Stimulation Leads to Akt/Protein Kinase B and Erk Activation in the Absence of the CD3-zeta Immune Receptor Tyrosine-based Activation Motifs. The Journal of Biological Chemistry 277, 13-22. Applications: Western Blotting
- Xu, H. and Goldfarb, M. (2001) J Biol Chem 276, 13049-56. Applications: Western Blotting
- Xing, H. et al. (2000) EMBO J 19, 349-58. Applications: Western Blotting
- Bolshakov, V.Y. et al. (2000) Nat Neurosci 3, 1107-12. Applications: Western Blotting
- Hisashi, N. et al. (2008) Nat Cell Biol 10, 971-8. Applications: Western Blotting
- Ohtani, M. et al. (2008) Blood 112, 635-43. Applications: Western Blotting
本製品は試験研究用です。