Cell Cycle / Checkpoint Control
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月7日15時25分 現在 |
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| #9110S | 10 mini-blots | 100,000 | ログインすると国内在庫状況がご確認いただけます。
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9110 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | アイソタイプ |
|---|---|---|---|---|---|
| Phospho-cdc2 (Tyr15) Antibody #9111 | 100 µl | W IP | H M R Mk Dm X | 34 | Rabbit |
| cdc2 Antibody #9112 | 100 µl | W | H M R | 34 | Rabbit |
| cdc2 (Tyr15) Control Proteins #9113 | 100 µl | 32, 34 | |||
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat | |||
| Anti-biotin, HRP-linked Antibody #7075 | 100 µl | Goat | |||
| Biotinylated Protein Ladder Detection Pack #7727 | 100 µl | ||||
| 20X LumiGLO® Reagent and 20X Peroxide #7003 | 5 ml |
| 貯法 | |
|---|---|
| -20℃ |
| MSDSはこちらから |
| 社内データ |
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Western Blotting
Western analysis of extracts from hydroxyurea (G1/S) or nocodizate (G2/M) treated Saos cells with Phospho-cdc2 (Tyr15) (A) and cdc2 (Tyr15) Antibodies (B).
| バックグラウンド |
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The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).
| 使用文献 |
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- Yu, C. et al. (2002) Pharmacologic mitogen-activated protein/extracellular signal-regulated kinase kinase/mitogen-activated protein kinase inhibitors interact synergistically with STI571 to induce apoptosis in Bcr/Abl-expressing human leukemia cells. Cancer Res. 62, 188-199. Applications: Western Blotting
- Dai, Y. et al. (2001) Pharmacological inhibitors of the mitogen-activated protein kinase (MAPK) kinase/MAPK cascade interact synergistically with UCN-01 to induce mitochondrial dysfunction and apoptosis in human leukemia cells. Cancer Res. 61, 5106-5115. Applications: Western Blotting
- Misaki, K. et al. (2001) PKN delays mitotic timing by inhibition of Cdc25C: possible involvement of PKN in the regulation of cell division. Proc. Natl. Acad. Sci. USA 98, 125-129. Applications: Western Blotting
本製品は試験研究用です。