Antibody Sampler Kits
PhosphoPlus® MEK1/2 (Ser217/221) Antibody Kit |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #9120S | 10 mini-blots | 100,000 | ログインすると国内在庫状況がご確認いただけます。
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9120 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | アイソタイプ |
|---|---|---|---|---|---|
| Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154 | 100 µl | W IP | H M R Mk Dm (C) | 45 | Rabbit IgG |
| MEK1/2 Antibody #9122 | 100 µl | W IP | H M R Mk Dm (C) (X) | 45 | Rabbit |
| MEK1/2 HeLa Control Cell Extracts #9160 | 4 mini-blots | ||||
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat | |||
| Anti-biotin, HRP-linked Antibody #7075 | 100 µl | Goat | |||
| 20X LumiGLO® Reagent and 20X Peroxide #7003 | 5 ml | ||||
| Biotinylated Protein Ladder Detection Pack #7727 | 100 µl |
| 貯法 | |
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| -20℃ |
| MSDSはこちらから |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from untreated or TPA-treated HeLa and NIH/3T3 cells, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154 (upper), or MEK1/2 Antibody #9122 (lower).
| バックグラウンド |
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MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
| 使用文献 |
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- Xiao, D. and Singh, S.V. (2002) Phenethyl isothiocyanate-induced apoptosis in p53-deficient PC-3 human prostate cancer cell line is mediated by extracellular signal-regulated kinases. Cancer Res. 62, 3615-3619. Applications: Western Blotting
- Watanabe, H. et al. (2001) Transcriptional cross-talk between Smad, ERK1/2, and p38 mitogen-activated protein kinase pathways regulates transforming growth factor-beta-induced aggrecan gene expression in chondrogenic ATDC5 cells. J. Biol. Chem. 276, 14466-14473. Applications: Western Blotting
- Jost, M. et al. (2001) Epidermal growth factor receptor-dependent control of keratinocyte survival and Bcl-xL expression through a MEK-dependent pathway. J. Biol. Chem. 276, 6320-6326. Applications: Western Blotting
- DeWindt, L. J. et al. (2000) Calcineurin promotes protein kinase C and c-Jun NH2-terminal kinase activation in the heart. Journal of Biological Chemistry 275, 13571-13579. Applications: Western Blotting
- Soga, S. et al. (1998) Radicicol leads to selective depletion of Raf kinase and disrupts K-Ras-activated aberrant signaling pathway. Journal of Biological Chemistry 273, 822-828. Applications: Western Blotting
- Galbiati, F. et al. (1998) Targeted downregulation of caveolin-1 is sufficient to drive cell transformation and hyperactivate the p42/44 MAP kinasecascade. European Molecular Biology Organization (EMBO) 17, 6633-6648. Applications: Western Blotting
本製品は試験研究用です。