MAP Kinase Signaling
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #9121S | 100 μL | 57,000 | |
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| #9121L | 300 μL | 137,000 | |
MEK1/2抗体製品一覧
9121 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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9121:
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Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:100） |
| 特異性・感度 | |
| 内在性レベルのSer217/221 がリン酸化されたMEK1/2 タンパク質を検出します。SEK (MKK4)、MKK3、MKK6を含む関連のキナーゼ タンパク質とは交差しません。またSer217 のみがリン酸化されたMEK1/2 タンパク質、Ser221 のみがリン酸化されたMEK1/2 タンパク質とも交差します。 |
| 使用抗原 | |
| ヒトのMEK1/2 タンパク質のSer217/221 周辺領域（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from untreated, TPA-treated or lambda phosphatase-treated HeLa cells, using Phospho-MEK 1/2 (Ser217/221) Antibody #9121 (upper) or MEK 1/2 Antibody #9122 (lower).
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
CST's Phospho- MEK1/2 (Ser217/221) Antibody selectively recognizes active MEK, i.e., only when phosphorylated at Ser217/221, and hence is an excellent marker of MEK1/2 activity.
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Crews, C.M. et al. (1992) Science 258, 478-480.
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Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
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Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
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Cowley, S. et al. (1994) Cell 77, 841-852.
- Piatelli, M.J. et al. (2004) Phosphatidylinositol 3-kinase-dependent mitogen-activated protein/extracellular signal-regulated kinase kinase 1/2 and NF-kappa B signaling pathways are required for B cell antigen receptor-mediated cyclin D2 induction in mature B cells. J. Immunol. 172, 2753-2762. Applications: Western Blotting
- Umenishi, F. and Schrier, R.W. (2003) Hypertonicity-induced aquaporin-1 (AQP1) expression is mediated by the activation of MAPK pathways and hypertonicity-responsive element in the AQP1 gene. J. Biol. Chem. 278, 15765-15770. Applications: Western Blotting
- Rosenberger, C.M. et al. (2002) Macrophages Inhibit Salmonella Typhimurium Replication through MEK/ERK Kinase and Phagocyte NADPH Oxidase Activities. The Journal of Biological Chemistry 277, 18753-18762. Applications: Western Blotting
- MacNicol, M.C. et al. (2000) Disruption of the 14-3-3 binding site within the B-Raf kinase domain uncouples catalytic activity from PC12 cell differentiation. J. Biol. Chem. 275, 3803-3809. Applications: Western Blotting
- Pizon, V. et al. (2000) Rap1A protein interferes with various MAP kinase activating pathways in skeletal myogenic cells. Oncogene 19, 6074-6081. Applications: Western Blotting
- Wang, X. et al. (2000) Requirement for ERK Activation in Cisplatin-induced Apoptosis. J. Biol. Chem. 275, 39435-39443. Applications: Western Blotting
- Oneyama, C. (2008) Mol Cell 30, 426-36. Applications: Western Blotting
PhosphoSitePlus®：
