MAP Kinase Signaling
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
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| #9121S | 100 μL | 57,000 | |
|
| #9121L | 300 μL | 137,000 | |
MEK1抗体製品一覧
推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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9121:
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Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:100） |
| 特異性・感度 | |
| 内在性レベルのSer217/221 がリン酸化されたMEK1/2 タンパク質を検出します。SEK (MKK4)、MKK3、MKK6を含む関連のキナーゼ タンパク質とは交差しません。またSer217 のみがリン酸化されたMEK1/2 タンパク質、Ser221 のみがリン酸化されたMEK1/2 タンパク質とも交差します。 |
| 使用抗原 | |
| ヒトのMEK1/2 タンパク質のSer217/221 周辺領域（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from untreated, TPA-treated or lambda phosphatase-treated HeLa cells, using Phospho-MEK 1/2 (Ser217/221) Antibody #9121 (upper) or MEK 1/2 Antibody #9122 (lower).
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
CST's Phospho- MEK1/2 (Ser217/221) Antibody selectively recognizes active MEK, i.e., only when phosphorylated at Ser217/221, and hence is an excellent marker of MEK1/2 activity.
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Crews, C.M. et al. (1992)
Science
258, 478-480.
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Alessi, D.R. et al. (1994)
EMBO J.
13, 1610-1619.
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Rosen, L.B. et al. (1994)
Neuron
12, 1207-1221.
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Cowley, S. et al. (1994)
Cell
77, 841-852.
- Piatelli, M.J. et al. (2004) Phosphatidylinositol 3-kinase-dependent mitogen-activated protein/extracellular signal-regulated kinase kinase 1/2 and NF-kappa B signaling pathways are required for B cell antigen receptor-mediated cyclin D2 induction in mature B cells. J. Immunol. 172, 2753-2762. Applications: Western Blotting
- Umenishi, F. and Schrier, R.W. (2003) Hypertonicity-induced aquaporin-1 (AQP1) expression is mediated by the activation of MAPK pathways and hypertonicity-responsive element in the AQP1 gene. J. Biol. Chem. 278, 15765-15770. Applications: Western Blotting
- Rosenberger, C.M. et al. (2002) Macrophages Inhibit Salmonella Typhimurium Replication through MEK/ERK Kinase and Phagocyte NADPH Oxidase Activities. The Journal of Biological Chemistry 277, 18753-18762. Applications: Western Blotting
- MacNicol, M.C. et al. (2000) Disruption of the 14-3-3 binding site within the B-Raf kinase domain uncouples catalytic activity from PC12 cell differentiation. J. Biol. Chem. 275, 3803-3809. Applications: Western Blotting
- Pizon, V. et al. (2000) Rap1A protein interferes with various MAP kinase activating pathways in skeletal myogenic cells. Oncogene 19, 6074-6081. Applications: Western Blotting
- Wang, X. et al. (2000) Requirement for ERK Activation in Cisplatin-induced Apoptosis. J. Biol. Chem. 275, 39435-39443. Applications: Western Blotting
- Oneyama, C. (2008) Mol Cell 30, 426-36. Applications: Western Blotting
