MAP Kinase Signaling
Phospho-MEK1 (Ser298) Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #9128S | 100 μL | 57,000 | |
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MEK1抗体製品一覧
9128 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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9128:
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Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000） |
| 特異性・感度 | |
| 内在性レベルのSer298 がリン酸化されたMEK1 タンパク質を検出します。リン酸化MEK2 とは交差しません。 |
| 使用抗原 | |
| ヒトのMEK1 タンパク質のSer298 周辺領域（合成リン酸化ペプチド） |
Western Blotting
Western blot analysis of extracts from C2C12, C6 and COS cells, untreated or PMA-treated, using Phospho-MEK1 (Ser298) Antibody (upper) or MEK1 Antibody #9124 (lower). In lane 3, the C2C12 lysate was treated with alkaline phosphatase (CIP) to show the phospho-specificity of the antibody.
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
MEK1 is phosphorylated at Ser298 by PAK1, which facilitates signal transduction from Raf to MEK1 and Erk2 (5-7). MEK1 is also phosphorylated by cdk5 at Thr286 in mitotic cells, causing negative feedback of the p44/42 MAP kinase pathway (8).
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Crews, C.M. et al. (1992) Science 258, 478-480.
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Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
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Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
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Cowley, S. et al. (1994) Cell 77, 841-852.
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Xu, B. et al. (1999) J. Biol. Chem. 274, 34029-34035.
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Coles, L.C. and Shaw, P.E. (2002) Oncogene 21, 2236-2244.
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Eblen, S. T. et al. (2002) Mol. Cell. Biol. 22, 6023-6033.
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Sharma, P. et al. (2002) J. Biol. Chem. 277, 528-534.
PhosphoSitePlus®：
MEK1