Jak/Stat Pathway
PhosphoPlus® Stat3 (Tyr705) Antibody Kit |
イイネ！(1) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日11時35分 現在 |
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| #9130S | 10 mini-blots | 100,000 | ログインすると国内在庫状況がご確認いただけます。
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9130 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 9132:
- ChIP Agarose ChIP Magnetic IHC / Paraffin Immunoprecipitation Western Blotting
- 9145:
- ChIP Agarose ChIP Magnetic Flow IHC / Frozen IHC / Paraffin Immunofluorescence* Immunoprecipitation Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | アイソタイプ |
|---|---|---|---|---|---|
| Stat3 Antibody #9132 | 100 µl | W IP IHC-P ChIP | H M R Mk (B) | 79, 86 | Rabbit |
| Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 | 100 µl | W IP IHC-P IHC-F IF-IC F ChIP | H M R Mk (Hm) (B) (Pg) (Hr) | 79, 86 | Rabbit IgG |
| Stat3 Control Cell Extracts #9133 | 40 µl | Human | |||
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat | |||
| Anti-biotin, HRP-linked Antibody #7075 | 100 µl | Goat | |||
| Biotinylated Protein Ladder Detection Pack #7727 | 100 µl | ||||
| 20X LumiGLO® Reagent and 20X Peroxide #7003 | 5 ml |
| 貯法 | |
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| -20℃ |
| MSDSはこちらから |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from IFN-α stimulated HeLa cells alone or with λ phosphatase treatment using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (left) or Stat3 Antibody #9132 (right).
| バックグラウンド |
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The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
- Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19, 75-120.
- Takeda, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 3801-3804.
- Catlett-Falcone, R. et al. (1999) Immunity 10, 105-115.
- Garcia, R. and Jove, R. (1998) J. Biomed. Sci. 5, 79-85.
- Bromberg, J.F. et al. (1999) Cell 98, 295-303.
- Darnell Jr., J.E. et al. (1994) Science 264, 1415-1421.
- Ihle, J.N. (1995) Nature 377, 591-594.
- Wen, Z. et al. (1995) Cell 82, 241-250.
- Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50.
- Biethahn, S. et al. (1999) Exp. Hematol. 27, 885-894.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。