Transcription Factors
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| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年9月9日14時20分 現在 |
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| #9131S | 100 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9131L | 300 μL | 137,000 |
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推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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| 用途 (希釈倍率) | |
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| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:100)、免疫蛍光細胞染色 (IF-IC) (1:100)、ChIP (1:50) | |
| 種交差性 | |
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| ヒト、マウス、ラット、サル、(ニワトリ、ウシ) | |
| 特異性・感度 | |
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| 内在性レベルのTyr705 がリン酸化されたStat3 タンパク質を検出します。類似のチロシン残基がリン酸化された他のStat タンパク質とは交差しませんが、リン酸化EGFR タンパク質とは交差します。 | |
| 検出タンパク質の分子量 | |
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| 79 kDa、86 kDa | |
| 使用抗原 | |
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| マウスのStat3 タンパク質のTyr705 周辺領域 (合成リン酸化ペプチド) | |
| 抗体の由来 | |
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| ウサギ | |
| 貯法 | |
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| -20℃ | |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from CNTF-treated SK-N-MC cells and EGF-treated A431 cells, using Phospho-Stat3 (Tyr705) Antibody (upper) or Stat3 Antibody #9132 (lower).
IP
Immunoprecipitation of extracts from CNTF-treated 35S-labeled SK-N-MC cells, using Phospho-Stat3 (Tyr705) Antibody (upper) or Stat3 Antibody #9132 (lower).
IF-IC
Confocal immunofluorescent analysis of HeLa cells either serum-starved (left) or IFNalpha-treated (right), using Phospho-Stat3 (Tyr705) Antibody (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red).
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes, and either 10 μl of Phospho-Stat3 (Tyr705) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP™ Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP™ Human c-Fos Promoter Primers #4663, and SimpleChIP™ Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
| バックグラウンド |
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The Stat3 transcription factor is an important signaling molecule for many cytokines and growth-factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
- Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19, 75-120.
- Takeda, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 3801-3804.
- Catlett-Falcone, R. et al. (1999) Immunity 10, 105-115.
- Garcia, R. and Jove, R. (1998) J. Biomed. Sci. 5, 79-85.
- Bromberg, J.F. et al. (1999) Cell 98, 295-303.
- Darnell Jr., J.E. et al. (1994) Science 264, 1415-1421.
- Ihle, J.N. (1995) Nature 377, 591-594.
- Wen, Z. et al. (1995) Cell 82, 241-250.
- Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50.
- Biethahn, S. et al. (1999) Exp. Hematol. 27, 885-894.
| 使用文献 |
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- Bartoli, M. et al. (2000) Vascular endothelial growth factor activates STAT proteins in aortic endothelial cells. J. Biol. Chem. 275, 33189-33192. Applications: IC-IF Western Blotting
- Campbell, C. L. et al. (2001) Increased expression of the interleukin-11 receptor and evidence of STAT3 activation of prostate carcinoma. Am. J. Path. 158, 25-32. Applications: Western Blotting
- Mitsui, K. et al. (2003) The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 113, 631-642. Applications: Western Blotting
- Guo, Z. et al. (2004) CDC91L1 (PIG-U) is a newly discovered oncogene in human bladder cancer. Nat. Med. 10, 374-381. Applications: IC-IF Western Blotting
- Butzkueven, H. et al. (2002) LIF receptor signaling limits immune-mediated demyelination by enhancing oligodendrocyte survival. Nat. Med. 8, 613-619. Applications: Western Blotting
- Bild, A. H. et al. (2002) Cytoplasmic transport of Stat3 by receptor-mediated endocytosis. EMBO J. 21, 3255-3263. Applications: Western Blotting
- Ducy, P. et al. (2000) Leptin inhibits bone formation through a hypothalamic relay: a central control of bone mass. Cell 100, 197-207. Applications: Western Blotting
- Fujimoto, M. et al. (2000) Defective thymocyte development and perturbed homeostasis of T cells in STAT-induced STAT inhibitor-1/suppressors of cytokine signaling-1 transgenic mice. J. Immunol. 165, 1799-1806. Applications: Western Blotting
- Hideshima, T. et al. (2001) The proteasome inhibitor PS-341 inhibits growth, induces apoptosis, and overcomes drug resistance in human multiple myeloma cells. Cancer Res. 61, 3071-3076. Applications: Western Blotting
- Weissenberger, J. et al. (2004) Oncogene 23, 3308-16. Applications: Western Blotting, IHC
- Cavallo, F. et al. (2001) Cancer Res 61, 3518-23. Applications: F
- Inoue, H. et al. (2004) Nat Med 10, 168-74. Applications: Western Blotting
- Park SJ. et al. (2004) J Immunol 173, 3844-54. Applications: Western Blotting
- Ohtani, M. et al. (2008) Blood 112, 635-43. Applications: Western Blotting
- Tanaka, Y. et al. (2008) Mol Cell Biol 28, 4805-18. Applications: Western Blotting
本製品は試験研究用です。
