Jak/Stat Pathway
- Jak/Stat Pathway
PhosphoSitePlus®：
STAT3
- 推奨プロトコール
Stat3 Antibody |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #9132S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9132L | 300 μL | 111,000 |
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9132 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:100)、免疫組織染色 (パラフィン) (1:100)、ChIP (1:25) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、(ウシ) |
| 特異性・感度 | |
|---|---|
| 内在性レベルのStat3 タンパク質を検出します。 |
| 検出タンパク質の分子量 | |
|---|---|
| 79 kDa、86 kDa |
| 使用抗原 | |
|---|---|
| マウスのStat3 タンパク質の配列 (合成ペプチド) |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or IFN-alpha treated (100 ng/ml for 5 min.), using Stat3 Antibody (left) or Phospho-Stat3 (Tyr705) Antibody #9131(right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using Stat3 Antibody.
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes, and either 10 μl of Stat3 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
| バックグラウンド |
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The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
- Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
- Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
- Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
- Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
- Bromberg, J.F. et al. (1999) Cell 98, 295-303.
- Darnell, J.E. et al. (1994) Science 264, 1415-21.
- Ihle, J.N. (1995) Nature 377, 591-4.
- Wen, Z. et al. (1995) Cell 82, 241-50.
- Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
- Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
| 使用文献 |
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- Bromberg, J. F. et al. (1999) Stat3 as an oncogene. Cell 98, 295-303. Applications: Western Blotting
- Craig, R. et al. (2000) p38 MAPK and NF-kappa B collaborate to induce interleukin-6 gene expression and release. J. Biol. Chem. 275, 23814-23824. Applications: IP Western Blotting
- Hutt, J. A. et al. (2000) Signal transducer and activator of transcription 3 activates CCAAT enhancer-binding protein delta gene transcription in G0 growth-arrested mouse mammary epithelial cells and in involuting mouse mammary gland. J. Biol. Chem. 275, 29123-29131. Applications: IP Western Blotting
- Peterson, W. M. et al. (2000) Ciliary neurotrophic factor and stress stimuli activate the Jak-STAT pathway in retinal neurons and glia. J. Neurosci. 20, 4081-4090. Applications: Western Blotting
- Ram, P. T. et al. (2000) Stat3-mediated transformation of NIH-3T3 cells by the constitutively active Q205L Galphao protein. Science 287, 142-144. Applications: Western Blotting
- Stairs, D.B. et al. (2011) Cancer Cell 19, 470-83. Applications: Western Blotting
- Mainardi, M. et al. (2010) Proc Natl Acad Sci U S A 107, 16673-8. Applications: IF-F
- Kumar, RN. et al. (2006) Oncogene 25, 899-906. Applications: IP, Western Blotting
- Catalano, RD. et al. (2005) Proc Natl Acad Sci U S A 102, 8585-90. Applications: Western Blotting
- Borowiak, M. (2004) Proc Natl Acad Sci U S A 101, 10608-13. Applications: Western Blotting
- Hor, S. et al. (2004) J Biol Chem 279, 33343-51. Applications: Western Blotting
- Woetmann, A. et al. (1999) Proc Natl Acad Sci U S A 96, 10620-5. Applications: IF, Western Blotting
- Park SJ. et al. (2004) J Immunol 173, 3844-54. Applications: Western Blotting
本製品は試験研究用です。