Tyrosine Kinases / Adaptors
Phospho-TrkA (Tyr490) Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #9141S | 100 μL | 57,000 | |
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| #9141L | 300 μL | 137,000 | |
TrkA抗体製品一覧
9141 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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9141:
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Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:100） |
| 特異性・感度 | |
| 内在性レベルのTyr490 がリン酸化されたTrkA タンパク質を検出します。また、同様の部位がリン酸化されたTrkB、TrkC タンパク質も検出する可能性があります。 |
| 使用抗原 | |
| ヒトのTrkA タンパク質のTyr490 周辺領域（合成ペプチド） |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of extracts from PC12 cells, untreated or NGF-treated (100 ng/ml), using Phospho-TrkA (Tyr490) Antibody.
The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).
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Pierotti, M.A. and Greco, A. (2006) Cancer Lett 232, 90-8.
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Segal, R.A. and Greenberg, M.E. (1996) Annu Rev Neurosci 19, 463-89.
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Stephens, R.M. et al. (1994) Neuron 12, 691-705.
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Obermeier, A. et al. (1993) EMBO J 12, 933-41.
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Obermeier, A. et al. (1994) EMBO J 13, 1585-90.
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Yao, R. and Cooper, G.M. (1995) Science 267, 2003-6.
- Anneren, C. et al. (2000) GTK, a Src-related tyrosine kinase, induces nerve growth factor-independent neurite outgrowth in PC12 cells through activation of the Rap1 pathway. J. Biol. Chem. 275, 29153-29161. Applications: Western Blotting
- Binder, D. K. et al. (1999) Immunohistochemical evidence of seizure-induced activation of trk receptors in the mossy fiber pathway of adult rat hippocampus. J. Neurosci. 19, 4616-4626. Applications: Western Blotting
- Gil, C. et al. (2000) Activation of signal transduction pathways involving trkA, PLCgamma-1, PKC isoforms and ERK-1/2 by tetanus toxin. FEBS Lett. 481, 177-182. Applications: Western Blotting
- Reuther, G. W. et al. (2000) Identification and characterization of an activating TrkA deletion mutation in acute myeloid leukemia. Mol. Cell. Biol. 20, 8655-8666. Applications: Western Blotting
- Binder, D. K. et al. (1999) Selective inhibition of kindling development by intraventricular administration of TrkB receptor body. J. Neurosci. 19, 1424-1436. Applications: Western Blotting
- Xu, B. et al. (2002) Neuroscience 115, 1295-308. Applications: Western Blotting
PhosphoSitePlus®：
TrkA