Jak/Stat Pathway
- Jak/Stat Pathway
PhosphoSitePlus®：
STAT3
- 推奨プロトコール
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb |
| イイネ！(9) |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #9145S | 100 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9145L | 300 μL | 137,000 | ||
| #9145P | 40 μL for Custom Sampler Kit |  Custom Antibody Sampler Kitの構成品を選択できます。 5本以上を選択し、ページ右上のCartから製品確定書を発行してください。 尚、構成品の単品販売は致しておりません。 |
シグナル伝達研究応援キャンペーン プレゼント *Pサイズのみ
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9145 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 9145:
- ChIP Agarose ChIP Magnetic Flow IHC / Frozen IHC / Paraffin Immunofluorescence* Immunoprecipitation Western Blotting
下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-F: 固定
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:2,000)、免疫沈降 (1:100)、免疫組織染色 (パラフィン) (1:400)、免疫組織染色 (凍結) (1:100)、免疫蛍光細胞染色 (IF-IC) (1:100)、フローサイトメトリー (1:200)、ChIP (1:100) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、(ウマ、ハムスター、ウシ、ブタ) |
| 特異性・感度 | |
|---|---|
| 内在性レベルのTyr705 がリン酸化されたStat3 タンパク質を検出します。リン酸化されたEGFR、あるいは類似のチロシン残基がリン酸化された他のStat タンパク質とは交差しません。 |
| 検出タンパク質の分子量 | |
|---|---|
| 79 kDa、86 kDa |
| 使用抗原 | |
|---|---|
| マウスのStat3 タンパク質のTyr705 周辺領域 (合成リン酸化ペプチド) |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from IFN-alpha treated Jurkat cells and HeLa cells (left), as well as EGF treated A431 cells (right), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb. Note that the basal phospho-Stat3 in A431 is detected by the antibody.
IHC-P (paraffin)
Immunohistochemical analysis using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb on SignalSlide (TM) Phospho-Stat 1/3/5 IHC Controls #8105 (paraffin-embedded 786-0 cells, untreated (left) or IFN-a treated (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Apc (min/+) mouse intestine, using Phosho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin embedded human breast carcinoma, specifically endothelial cells, untreated (left) or lambda phosphatase treated (right), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.
IHC-P (paraffin)
Immunnohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localization, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.
IHC-F (frozen)
Immunohistochemical analysis of frozen H1650 xenograft using Phospho-Stat3 (Tyr705)(D3A7) XP® Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of HeLa cells, untreated (blue) or IFN-α treated (green), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells, IFN-alpha treated (left) or untreated (right), labeled with Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (green).
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes, and either 10 μl of Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
| バックグラウンド |
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The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
- Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
- Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
- Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
- Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
- Bromberg, J.F. et al. (1999) Cell 98, 295-303.
- Darnell, J.E. et al. (1994) Science 264, 1415-21.
- Ihle, J.N. (1995) Nature 377, 591-4.
- Wen, Z. et al. (1995) Cell 82, 241-50.
- Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
- Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
| 使用文献 |
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- Tripathi, R.B. and McTigue, D.M. (2008) J Comp Neurol 510, 129-44. Applications: IC-IF
- Ernst, M.B. et al. (2009) J Neurosci 29, 11582-93. Applications: IHC-F (floating)
- Bauer, S. and Patterson, P.H. (2006) J Neurosci 26, 12089-99. Applications: IHC-FL (floating/frozen)
- Yao, Z. et al. (2010) Proc Natl Acad Sci U S A 107, 15535-40. Applications: Western Blotting
- Surdziel, E. et al. (2011) Blood 117, 4338-48. Applications: Western Blotting
- Stairs, D.B. et al. (2011) Cancer Cell 19, 470-83. Applications: IC-IF
- Anand, S. et al. (2011) Blood 118, 1610-21. Applications: Western Blotting
- Mainardi, M. et al. (2010) Proc Natl Acad Sci U S A 107, 16673-8. Applications: IF-F
- Matsuyama, H. et al. (2011) Blood 118, 6881-92. Applications: Western Blotting
本製品は試験研究用です。