Jak/Stat Pathway
- Jak/Stat Pathway
PhosphoSitePlus®：
STAT1
- 推奨プロトコール
Phospho-Stat1 (Tyr701) Antibody |
イイネ！(4) |
|
|
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
|
|---|---|---|---|---|
| #9171S | 100 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
| |
| #9171L | 300 μL | 137,000 | ||
| #9171P | 40 μL for Custom Sampler Kit |  Custom Antibody Sampler Kitの構成品を選択できます。 5本以上を選択し、ページ右上のCartから製品確定書を発行してください。 尚、構成品の単品販売は致しておりません。 |
シグナル伝達研究応援キャンペーン プレゼント *Pサイズのみ
|
9171 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
|---|
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:100)、ChIP (1:25) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、(ウシ、イヌ) |
| 特異性・感度 | |
|---|---|
| 内在性レベルのTyr701 がリン酸化されたStat1 タンパク質を検出します。Tyr701 がリン酸化されたp91 Stat1 とp84 スプライシング変異タンパク質も検出します。チロシンがリン酸化した他のStat タンパク質とは交差しません。 |
| 検出タンパク質の分子量 | |
|---|---|
| 84 kDa、91 kDa |
| 使用抗原 | |
|---|---|
| ヒトのStat1 タンパク質のTyr701 周辺領域 (合成リン酸化ペプチド) |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
|---|
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or IFN-alpha-treated (100 ng/ml), using Phospho-Stat1 (Tyr701) Antibody.
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either 20 μl of Phospho-Stat1 (Tyr701) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
| バックグラウンド |
|---|
The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
| 使用文献 |
|---|
- Begitt, A. et al. (2000) Nucleocytoplasmic translocation of Stat1 is regulated by a leucine-rich export signal in the coiled-coil domain. Proc. Natl. Acad. Sci. USA 97, 10418-10423. Applications: Western Blotting
- Gamero, A.M. and Larner, A.C. (2000) Signaling via the T cell antigen receptor induces phosphorylation of Stat1 on serine 727. J. Biol. Chem. 275, 16574-16578. Applications: Western Blotting
- McBride, K. M. et al. (2000) Nuclear export signal located within the DNA-binding domain of the STAT1 transcription factor. EMBO J. 19, 6196-6206. Applications: Western Blotting
- Spagnoli, A. et al. (2002) Identification of STAT-1 as a molecular target of IGFBP-3 in the process of chondrogenesis. J. Biol. Chem. 277, 18860-18867. Applications: IC-ABC Western Blotting
- Hirata, A. et al. (2002) ZD1839 (Iressa) induces antiangiogenic effects through inhibition of epidermal growth factor receptor tyrosine kinase. Cancer Res. 62, 2554-2560. Applications: Western Blotting
- Hayashi, T. et al. (2002) Regulation of receptor activator of NF-kappaB ligand-induced osteoclastogenesis by endogenous interferon-beta (INF-beta) and suppressors of cytokine signaling (SOCS). J. Biol. Chem. 277, 27880-27886. Applications: Western Blotting
- Mowen, K. A. et al. (2001) Arginine methylation of STAT1 modulates IFN alpha/beta-induced transcription. Cell 104, 731-741. Applications: Western Blotting
- Teague, T. K. et al. (2000) Activation-induced inhibition of interleukin 6-mediated T cell survival and signal transducer and activator of transcription 1 signaling. J. Exp. Med. 191, 915-926. Applications: Western Blotting
- Lee, J. et al. (2011) Oncogene Applications: Western Blotting
- Zimnik, S. et al. (2009) Nucleic Acids Res 37, e30. Applications: Western Blotting
- Takagi, Z. et al.(2005) J Immunol 174, 1801-10. Applications: Western Blotting, IP
- Zhang, Y. et al. (2005) J Biol Chem 280, 34306-15. Applications: Western Blotting
- Hor, S. et al. (2004) J Biol Chem 279, 33343-51. Applications: Western Blotting
- Bhanoori, M. et al. (2003) Oncogene 922, 117-30. Applications: Western Blotting
- Abe, T. et al. (2007) J Virol 81, 8953-66. Applications: Western Blotting
本製品は試験研究用です。