Neuroscience
- Neuroscience
PhosphoSitePlus®：
CREB
- 推奨プロトコール
CREB (48H2) Rabbit mAb |
イイネ！(2) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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|---|---|---|---|---|
| #9197S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9197L | 300 μL | 111,000 |
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9197 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 9197:
- ChIP Agarose ChIP Magnetic Flow IHC / Frozen IHC / Paraffin Immunofluorescence Immunoprecipitation Western Blotting
下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-F: 固定
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:250)、免疫組織染色 (パラフィン) (1:6,400)、免疫組織染色 (凍結) (1:6,400)、免疫蛍光染色 (IF-F) (1:1,600)、免疫蛍光細胞染色 (IF-IC) (1:1,600)、フローサイトメトリー (1:400)、ChIP (1:50) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、キイロショウジョウバエ |
| 特異性・感度 | |
|---|---|
| 内在性レベルのCREB タンパク質を検出します。 |
| 検出タンパク質の分子量 | |
|---|---|
| 43 kDa |
| 使用抗原 | |
|---|---|
| ヒトのCREB タンパク質のN末端領域 (組換えタンパク質) |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| 社内データ |
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Western Blotting
Western Blot analysis of extracts from SK-N-MC, COS, NIH/3T3, C6 and Drosophila S2 cells, using CREB (48H2) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human astrocytoma, using CREB (48H2) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using CREB (48H2) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using CREB (48H2) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse brain, using CREB (48H2) Rabbit mAb.
IHC-F (frozen)
Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using CREB (48H2) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of SK-N-MC cells using CREB (48H2) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of SK-N-MC cells showing nuclear stain with CREB (48H2) Rabbit mAb (A, red) compared to an isotype control (B). Blue pseudocolor =DRAQ5® (fluorescent DNA dye).
IF-F
Confocal immunofluorescent analysis of mouse cerebellum labeled with CREB (48H2) Rabbit mAb (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor =DRAQ5® #4084 (fluorescent DNA dye).
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells, treated with Forskolin #3828 (30 μM) for 1h and either 10 μl of CREB (48H2) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
| バックグラウンド |
|---|
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).
- Lonze, B.E. et al. (2002) Neuron 34, 371-85.
- Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
- Redmond, L. et al. (2002) Neuron 34, 999-1010.
- Dash, P.K. et al. (1990) Nature 345, 718-21.
- Yin, J.C. et al. (1994) Cell 79, 49-58.
- Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
- Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
- Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
- Tan, Y. et al. (1996) EMBO J 15, 4629-42.
| 使用文献 |
|---|
- Banno, Y. et al. (2008) J Neurochem 104, 1372-86. Applications: Western Blotting
- Kumar, A.P. et al. (2007) Clin Cancer Res 13, 2784-94. Applications: IHC-P (paraffin)
- Ghosh, R. et al. (2007) Neoplasia 9, 893-9. Applications: IHC-P (paraffin)
- Sakaguchi, M. et al. (2008) Mol Biol Cell 19, 78-85. Applications: Western Blotting
- Gaddini, L. et al. (2009) Neurobiol Dis 35, 278-85. Applications: Western Blotting
- Almeida, L.E. et al. (2009) J Neurosci 29, 12702-10. Applications: Western Blotting
- Gehani, S.S. et al. (2010) Mol Cell 39, 886-900. Applications: Western Blotting
- Chung, Y.W. et al. (2011) J Biol Chem 286, 29681-90. Applications: Western Blotting
- Suzuki, S. et al. (2007) J Biol Chem 282, 25177-81. Applications: Western Blotting
本製品は試験研究用です。