MAP Kinase Signaling
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月7日15時25分 現在 |
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| #9210S | 20 mini-blots | 100,000 | ログインすると国内在庫状況がご確認いただけます。
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9210 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 4511:
- Flow IHC / Paraffin Immunofluorescence Immunoprecipitation Western Blotting
- 9212:
- Flow IHC / Paraffin Immunofluorescence Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | アイソタイプ |
|---|---|---|---|---|---|
| Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 | 200 µl | W IP IHC-P IF-IC F | H M R Mk Sc (Hm) (C) (Z) (B) (Pg) | 43 | Rabbit IgG |
| p38 MAPK Antibody #9212 | 200 µl | W IHC-P IF-IC F | H M R Mk GP (C) | 43 | Rabbit |
| p38 MAPK Control Cell Extracts #9213 | 60 µl | ||||
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat | |||
| Anti-biotin, HRP-linked Antibody #7075 | 250 µl | Goat | |||
| Biotinylated Protein Ladder Detection Pack #7727 | 250 µl | ||||
| 20X LumiGLO® Reagent and 20X Peroxide #7003 | 10 ml |
| 貯法 | |
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| -20℃ |
| MSDSはこちらから |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from COS and 293 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 (upper) or p38 MAPK Antibody #9212 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAPK Antibody #9212.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using p38 MAPK Antibody #9212 (blue) compared to a nonspecific negative control antibody (red).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or anisomycin-treated (green), using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 compared to a nonspecific negative control antibody (red).
| バックグラウンド |
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p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
- Rouse, J. et al. (1994) Cell 78, 1027-1037.
- Han, J. et al. (1994) Science 265, 808-811.
- Lee, J.C. et al. (1994) Nature 372, 739-746.
- Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
- Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
- Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
- Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
- Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
- Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.
| 使用文献 |
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- Liu, F. et al. (2002) GnRH activates ERK1/2 leading to the induction of c-fos and LHbeta protein expression in LbetaT2 cells. Mol. Endocrinol. 16, 419-434. Applications: Western Blotting
- Legos, J. J. et al. (2001) SB 239063, a novel p38 inhibitor, attenuates early neuronal injury following ischemia. Brain Research 892, 70-77. Applications: Western Blotting
- Watanabe, H. et al. (2001) Transcriptional Cross-talk between Smad, ERK1/2, andp38 Mitogen-activated Protein Kinase Pathways Regulates Transforming Growth Factor-beta -induced Aggrecan Gene Expression in ChondrogenicATDC5 Cells. Journal of Biological Chemistry 276, 14466-14473. Applications: Western Blotting
- De, A. K. et al. (2000) Exaggerated human monocyte IL-10 concomitant to minimal TNF-alpha induction by heat-shock protein 27 (Hsp27) suggests Hsp27 is primarily an antiinflammatory stimulus [In ProcessCitation]. Journal of Immunology 165, 3951-3958. Applications: Western Blotting
- Lee, Y. W. et al. (2000) 2-Amino-3-methylimidazo[4,5-f]quinoline inhibits nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 cells by blocking p38 kinase activation. Cancer Lett. 156, 133-139. Applications: Western Blotting
- Griego, S. D. et al. (2000) Role of p38 mitogen-activated protein kinase in rhinovirus-induced cytokine production by bronchial epithelial cells[In Process Citation]. Journal of Immunology 165, 5211-5220. Applications: Western Blotting
- Novitskaya, V. et al. (2000) Oligomeric forms of the metastasis related Mts1(S100A4) protein, stimulate neuronal differentiation in cultures of rat hippocampal neurons. Journal of Biological Chemistry Applications: Western Blotting
本製品は試験研究用です。