MAP Kinase Signaling
- MAP Kinase Signaling
PhosphoSitePlus®：
p38-alpha
- 推奨プロトコール
推奨アプリケーションで優れたパフォーマンスを発揮するXP®モノクローナル抗体をお試しください : 4511
Phospho-p38 MAPK (Thr180/Tyr182) Antibody |
イイネ！(4) |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
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| #9211S | 200 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9211L | 600 μL | 137,000 |
MAPKp38 XP®モノクローナル抗体 | MAPKp38抗体製品一覧
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9211 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50)、免疫蛍光細胞染色 (IF-IC) (1:200)、フローサイトメトリー (1:200) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、キイロショウジョウバエ、ブタ、出芽酵母、 (ハムスター、ウシ、ゼブラフィッシュ) |
| 特異性・感度 | |
|---|---|
| 内在性レベルのThr180/Tyr182 がリン酸化されたp38 MAPK タンパク質を検出します。リン酸化されたp44/42 MAPK およびSAPK/JNK タンパク質とは交差しません。また、Thr180 だけ、あるいはTyr182 だけがリン酸化されたp38 とも交差します。 |
| 検出タンパク質の分子量 | |
|---|---|
| 43 kDa |
| 使用抗原 | |
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| ヒトのp38 MAPK タンパク質のThr180/Tyr182 周辺領域 (合成リン酸化ペプチド) |
| 抗体の由来 | |
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| ウサギ |
| 貯法 | |
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| -20℃ |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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推奨アプリケーションで優れたパフォーマンスを発揮するXP®モノクローナル抗体をお試しください : 4511
Western Blotting
Western blot analysis of extracts from C6 cells, untreated or anisomycin-treated, and NIH/3T3 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody (upper) or p38 MAPK Antibody #9212 (lower).
Western Blotting
Western blot analysis of extracts from UV-treated NIH/3T3 cells, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody (upper) or control p38 MAPK Antibody #9212 (lower).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or anisomycin treated (blue), using Phospho-p38 MAPK (Thr180/Tyr182) Antibody compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells -/+ UV light, labeled with Phospho-p38 MAPK (green). Absence of staining in untreated cells (left) and nuclear localization in treated cells (right). Red = Actin filaments (phalloidin).
| バックグラウンド |
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p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
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| 使用文献 |
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- Yang, P. et al. (2010) Nat Immunol 11, 487-94. Applications: Western Blotting
- Kawagoe, T. et al. (2008) Nat Immunol 9, 684-91. Applications: Western Blotting
- Herczenik, E. et al. (2007) Arterioscler Thromb Vasc Biol 27, 1657-65. Applications: Western Blotting
- Zaru, R. et al. (2007) Nat Immunol 8, 1227-35. Applications: Western Blotting
- Hsu, J.T. et al. (2007) Am J Physiol Heart Circ Physiol 292, H2982-7. Applications: Western Blotting
- Goss, V.L. et al. (2006) Blood 107, 4888-97. Applications: Western Blotting
- Matsumoto, M. et al. (2004) J Biol Chem 279, 45969-79. Applications: IF-IC (In Cells)
- Patrucco, E. et al. (2004) PI3Kgamma modulates the cardiac response to chronic pressure overload by distinct kinase-dependent and -independent effects. Cell 118, 375-387. Applications: Western Blotting
- Hata, K. et al. (2003) Differential roles of Smad1 and p38 kinase in regulation of peroxisome proliferator-activating receptor gamma during bone morphogenetic protein 2-induced adipogenesis. Mol. Biol. Cell 14, 545-555. Applications: Western Blotting
- Liu, F. et al. (2002) GnRH activates ERK1/2 leading to the induction of c-fos and LHbeta protein expression in LbetaT2 cells. Mol. Endocrinol. 16, 419-434. Applications: Western Blotting
- Xing, H. et al. (2000) 14-3-3 proteins block apoptosis and differentially regulate MAPK cascades. EMBO J. 19, 349-358. Applications: Western Blotting
- Villunger, A. et al. (2000) Fas ligand, Bcl-2, granulocyte colony-stimulating factor, and p38 mitogen-activated protein kinase: regulators of distinct cell death and survival pathways in granulocytes. J. Exp. Med. 192, 647-658. Applications: Western Blotting
- Lee, Y. W. et al. (2000) 2-Amino-3-methylimidazo[4,5-f] quinoline inhibits nitric oxide production in lipopolysaccharide-stimulated RAW 264. Cancer Lett. 156, 133-139. Applications: Western Blotting
- Uddin, S. et al. (2000) The Rac1/p38 mitogen-activated protein kinase pathway is required for interferon alpha-dependent transcriptional activation but not serine phosphorylation of Stat proteins. J. Biol. Chem. 275, 27634-27640. Applications: Western Blotting
- Kojima, R. et al. (2004) Biochem J 380, 783-94. Applications: Western Blotting
- Ohtani, M. et al. (2008) Blood 112, 635-43. Applications: Western Blotting
- Arimoto, K. et al. (2008) Nat Cell Biol 10, 1324-32. Applications: Western Blotting
- Kubo, T. et al. (2009) J Exp Med 206, 1971-82. Applications: Western Blotting
- Nishimura, M. et al. (2009) Mol Cell Biol 29, 5529-39. Applications: Western Blotting
- Suzuki, S. et al. (2007) J Biol Chem 282, 25177-81. Applications: Western Blotting
本製品は試験研究用です。