MAP Kinase Signaling
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PhosphoSitePlus®：
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月3日18時20分 現在 |
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|---|---|---|---|---|
| #9212S | 200 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9212L | 600 μL | 111,000 | ||
| #9212P | 40 μL for Custom Sampler Kit |  Custom Antibody Sampler Kitの構成品を選択できます。 5本以上を選択し、ページ右上のCartから製品確定書を発行してください。 尚、構成品の単品販売は致しておりません。 |
シグナル伝達研究応援キャンペーン プレゼント *Pサイズのみ
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9212 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫組織染色 (パラフィン) (1:200)、免疫蛍光細胞染色 (IF-IC) (1:50)、フローサイトメトリー (1:50) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、モルモット、(ニワトリ) |
| 特異性・感度 | |
|---|---|
| 内在性レベルのp38α、p38β、p38γ MAPK タンパク質を検出します。p38δ MAPK、JNK/SAPK、あるいはp44/42 MAPK タンパク質は認識しません。 |
| 検出タンパク質の分子量 | |
|---|---|
| 43 kDa |
| 使用抗原 | |
|---|---|
| ヒトのp38 MAPK タンパク質由来の配列 (合成ペプチド) |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® p38 MAPK siRNA I #6564 (+) or SignalSilence® p38 MAPK siRNA II (+), using p38 MAPK Antibody #9212 and α-Tubulin (11H10) Rabbit mAb #2125. The p38 MAPK antibody confirms silencing of p38 MAPK expression while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of p38 MAPK siRNA.
Western Blotting
Western blot analysis of extracts from C6 cells, untreated or anisomycin-treated, and NIH/3T3 cells, untreated or UV-treated, using phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211 (upper) or p38 MAPK Antibody (lower).
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or UV-treated for the indicated times, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211 (upper) or p38 MAPK Antibody (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAPK Antibody.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using p38 MAPK Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent images of HeLa cells-/+ UV light labeled with #9212 p38 MAPK (green) exhibiting cytoplasmic localization in untreated cells (left) and nuclear localization in treated cells (right). Red = Actin filaments (phalloidin).
| バックグラウンド |
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p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
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- Han, J. et al. (1994) Science 265, 808-811.
- Lee, J.C. et al. (1994) Nature 372, 739-746.
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- Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
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- Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
- Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.
| 使用文献 |
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- Miyaji, M. et al. (2009) Proc Natl Acad Sci U S A 106, 14502-7. Applications: Western Blotting
- Hsu, J.T. et al. (2007) Am J Physiol Heart Circ Physiol 292, H2982-7. Applications: Western Blotting
- Kojima, R. et al. (2004) Biochem J 380, 783-94. Applications: Western Blotting
- Matsumoto, M. et al. (2004) J Biol Chem 279, 45969-79. Applications: IF-IC (In Cells)
- Matsumoto, M. et al. (2000) Involvement of p38 mitogen-activated protein kinase signaling pathway in osteoclastogenesis mediated by receptor activator of NF-kappa B ligand (RANKL). J. Biol. Chem. 275, 31155-31161. Applications: Western Blotting
- Chen, C. H. et al. (2000) Cyclic AMP activates p38 mitogen-activated protein kinase in Th2 cells: phosphorylation of GATA-3 and stimulation of Th2 cytokine gene expression. J. Immunol. 165, 5597-5605. Applications: Western Blotting
- Mudgett, J. S. et al. (2000) Essential role for p38alpha mitogen-activated protein kinase in placental angiogenesis. Proc. Natl. Acad. Sci. USA 97, 10454-10459. Applications: IHC-P (paraffin) Western Blotting
- Rossi, A. et al. (2000) Anti-inflammatory cyclopentenone prostaglandins are direct inhibitors of IkappaB kinase. Nature 403, 103-108. Applications: Western Blotting
- Zhuang, S. et al. (2000) p38 mitogen-activated protein kinase mediates bid cleavage, mitochondrial dysfunction, and caspase-3 activation during apoptosis induced by singlet oxygen but not by hydrogen peroxide. J. Biol. Chem. 275, 25939-25948. Applications: Western Blotting
- Rohani, M.G. et al. (2010) BMC Immunol 11, 53. Applications: Western Blotting
- Gehani, S.S. et al. (2010) Mol Cell 39, 886-900. Applications: Western Blotting
- Lee, W.H. et al. (2011) J Biol Chem 286, 21577-87. Applications: Western Blotting
本製品は試験研究用です。