MAP Kinase Signaling
p38δ MAPK Antibody
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| #9214S | 100 μL | 46,000 | |
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MAPKp38-delta抗体製品一覧
9214 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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9214:
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Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000） |
| 特異性・感度 | |
| 内在性レベルのp38δ MAPK タンパク質を検出します。p38 MAPK タンパク質の他のアイソフォームとは交差しません。 |
| 使用抗原 | |
| p38δ MAPK タンパク質のC末端近傍配列（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from HEK293, THP1 and Ramos cells, using p38δ MAPK Antibody.
Western Blotting
Western blot analysis of extracts from Xenopus oocytes overexpressing Myc-tagged p38 isoforms, using p38δ MAPK Antibody (upper) and Myc-tag antibody (lower). Results show the isoform specificity of CST's p38δ MAPK Antibody. (Provided by Dr. Eusebio Perdiguero and Dr. Angel Nebreda, European Molecular Biology Laboratory, Germany.)
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
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Rouse, J. et al. (1994) Cell 78, 1027-1037.
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Han, J. et al. (1994) Science 265, 808-811.
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Lee, J.C. et al. (1994) Nature 372, 739-746.
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Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
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Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
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Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
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Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
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Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
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Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.
PhosphoSitePlus®：
p38-delta