MAP Kinase Signaling
Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb (Sepharose Bead Conjugate)
|
イイネ！(0)
| 
|
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
ご登録代理店情報 カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更は こちら。 |
| #9219S | 800 μL | 61,000 | |
|
MAPKp38抗体製品一覧
9219 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
| | |
-
9219:
-
Immunoprecipitation
| 特異性・感度 | |
| 内在性レベルのThr180/Tyr182 がリン酸化されたp38 MAPK と結合します。リン酸化されたSAPK/JNK およびp44/42 MAPK （Erk1/2）、リン酸化されていないp38 MAPK タンパク質とは有意に交差しません。 |
| 使用抗原 | |
| ヒトのp38 MAP Kinase タンパク質のThr180/Tyr182 周辺領域（合成リン酸化ペプチド） |
IP
p38 MAPK activity of UV-treated or untreated C6 cell extracts was analyzed by IP/kinase assay. Cell extracts were immunoprecipitated with Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb (Sepharose Bead Conjugate). In vitro kinase assays were performed using ATF-2 Fusion Protein #9224 as a substrate. Phosphorylation of ATF-2 at Thr71 was measured by Western blot using Phospho-ATF-2 (Thr71) Antibody.
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
-
Rouse, J. et al. (1994) Cell 78, 1027-1037.
-
Han, J. et al. (1994) Science 265, 808-811.
-
Lee, J.C. et al. (1994) Nature 372, 739-746.
-
Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
-
Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
-
Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
-
Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
-
Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
-
Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
-
Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.
PhosphoSitePlus®：
p38-alpha