#9224 ATF-2 Fusion Protein
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年5月18日15時45分 現在 |
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| #9224S | 0.1 mg | 22,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9224L | 0.5 mg | 85,000 |
| 概要 | |
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| ATF-2 タンパク質の19〜96 番目の残基を含む組換えタンパク質 |
| 由来 | |
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| ヒトのcDNA ライブラリーからクローン化し、大腸菌を用いて過剰発現させたもの |
| 見かけの分子量 | |
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| 34 kDa |
| 社内データ |
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Gel Staining
Figure 1. Western blot analysis of ATF-2 fusion protein phosphorylated by different isoforms of p38 kinase, using ATF-2 (20F1) Rabbit mAb #9226 (upper panel) and Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb #5112 (lower panel).
Kinase Assay - Radiometric
Figure 2. ATF-2 fusion protein was used as substrate to measure p38 kinases activity in a radiometric assay using the following reaction conditions: 25 mM Tris-HCl (pH7.5), 10 mM MgCl2, 5 mM b-glycerophosphate, 0.1 mM Na3VO4, 2 mM DTT, 50 μM ATP, Substrate: ATF-2 fusion protein 400 ng/μL, and p38 kinases: 100 ng/25 μL.
| バックグラウンド |
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The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
| 使用文献 |
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- Heuertz, R. M. et al. (1999) C-reactive protein inhibits chemotactic peptide-induced p38 mitogen-activated protein kinase activity and human neutrophil movement. Journal of Biological Chemistry 274, 17968-17974. Applications: kinase assay substrate
- Page, K. et al. (1999) Platelet-derived growth factor stimulation of mitogen-activated protein kinases and cyclin D1 promoter activity in cultured airway smooth-muscle cells. Role of Ras. American Journal of Respiratory Cell and Molecular Biology 20, 1294-1302. Applications: kinase assay substrate
- Rao, G.N. (2000) Oxidant stress stimulates phosphorylation of eIF4E without an effect on global protein synthesis in smooth muscle cells. Lack of evidence for a role of H202 in angiotensin II-induced hypertrophy. Journal of Biological Chemistry 275, 16993-16999. Applications: kinase assay substrate
本製品は試験研究用です。
