MAP Kinase Signaling
PhosphoPlus® SAPK/JNK (Thr183/Tyr185) Antibody Kit |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #9250S | 20 mini-blots | 100,000 | ログインすると国内在庫状況がご確認いただけます。
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9250 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 9251:
- IHC / Paraffin Immunoprecipitation Western Blotting
- 9253:
- Western Blotting
- 9258:
- Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | アイソタイプ |
|---|---|---|---|---|---|
| Phospho-SAPK/JNK (Thr183/Tyr185) Antibody #9251 | 200 µl | W IP IHC-P | H M R Hm Mk Dm B Sc (X) | 46, 54 | Rabbit |
| SAPK/JNK (56G8) Rabbit mAb #9258 | 200 µl | W | H M R Hm Mk Mi | 46, 54 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat | |||
| Anti-biotin, HRP-linked Antibody #7075 | 250 µl | Goat | |||
| 20X LumiGLO® Reagent and 20X Peroxide #7003 | 10 ml | ||||
| Biotinylated Protein Ladder Detection Pack #7727 | 250 µl | ||||
| SAPK/JNK Control Cell Extracts #9253 | 60 µl |
| 貯法 | |
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| -20℃ |
| MSDSはこちらから |
| 社内データ |
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Western Blotting
Phospho SAPK/JNK (Thr183/Tyr185) Antibody recognizes p54/p46 SAPK/JNK but not Erk1/2 or p38 MAPK. Western blot analysis of C6 cell extracts with and without anisomycin treatment, using Phospho-ERK1/2 Antibody #9101 (left), Phospho-SAPK/JNK Antibody #9251 (center) and Phospho-p38 MAPK Antibody #9211 (right).
| バックグラウンド |
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The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).
- Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
- Ichijo, H. (1999) Oncogene 18, 6087-93.
- Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
- Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
- Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
- Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.
| 使用文献 |
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本製品は試験研究用です。