Wnt / Hedgehog / Notch
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月7日15時25分 現在 |
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|---|---|---|---|---|
| #9331S | 100 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9331L | 300 μL | 137,000 |
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9331 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途（希釈倍率） | |
|---|---|
| ウェスタンブロッティング（1:1,000）、免疫組織染色（パラフィン）（1:50） |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、キイロショウジョウバエ、ゼブラフィッシュ |
| 特異性・感度 | |
|---|---|
| 内在性レベルのSer21 がリン酸化されたGSK-3α タンパク質およびSer9 がリン酸化されたGSK-3β タンパク質を検出します。 |
| 検出タンパク質の分子量 | |
|---|---|
| 46 kDa、51 kDa |
| 使用抗原 | |
|---|---|
| ヒトのGSK-3α タンパク質の配列（合成リン酸化ペプチド） |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from 293 cells, untreated or insulin treated for the indicated times, using Phospho-GSK-3alpha/beta (Ser21/9) Antibody (upper) or GSK-3beta Antibody #9332 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using Phospho-GSK-3alpha/beta (Ser21/9) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-GSK-3alpha/beta (Ser21/9) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-GSK-3alpha/beta (Ser21/9) Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right), using Phospho-GSK-3alpha/beta (Ser21/9) Antibody. Note the reduction of stain in the LY294002-treated cells.
| バックグラウンド |
|---|
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
| 使用文献 |
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- Armstrong, J. L. et al. (2001) Regulation of glycogen synthesis by amino acids in cultured human muscle cells. J. Biol. Chem. 276, 952-956. Applications: Western Blotting
- Fasshauer, M. et al. (2000) Essential role of insulin receptor substrate-2 in insulin stimulation of Glut4 translocation and glucose uptake in brown adipocytes. J. Biol. Chem. 275, 25494-25501. Applications: Western Blotting
- Zheng, W. H. et al. (2000) Insulin-like growth factor-1-induced phosphorylation of the Forkhead family transcription factor FKHRL1 Is mediated by Akt kinase in PC12 cells. J. Biol. Chem. 275, 39152-39158. Applications: Western Blotting
- Peyssonnaux, C. et al. (2000) Induction of postmitotic neuroretina cell proliferation by distinct Ras downstream signaling pathways. Mol. Cell. Biol. 20, 7068-7079. Applications: Western Blotting
- Allard, D. et al. (2008) J Biol Chem 283, 19739-47. Applications: Western Blotting
- Popkie, A.P. et al. (2010) J Biol Chem 285, 41337-47. Applications: Western Blotting
- Fonseca, B.D. et al. (2011) J Biol Chem 286, 27111-22. Applications: Western Blotting
- Ohtani, M. et al. (2008) Blood 112, 635-43. Applications: Western Blotting
- Kitamura, T. et al. (2008) Nat Cell Biol 10, 329-37. Applications: Western Blotting
本製品は試験研究用です。