Jak/Stat Pathway
Phospho-Stat5 (Tyr694) Antibody
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イイネ！(6)
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| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #9351S | 100 μL | 57,000 | |
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| #9351L | 300 μL | 137,000 | |
Stat5抗体製品一覧
9351 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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9351:
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ChIP Agarose
ChIP Magnetic
Flow
Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000)、フローサイトメトリー (1:400)、ChIP (1:50) |
| 特異性・感度 | |
| 内在性レベルのTyr694 がリン酸化されたStat5a およびTyr699 がリン酸化されたStat5b タンパク質を検出します。同等のチロシンがリン酸化された他のStat タンパク質とは交差しませんが、EGFR タンパク質とは交差します。 |
| 使用抗原 | |
| マウスのStat5a タンパク質のTyr694 周辺領域 (合成リン酸化ペプチド) |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of extracts from TF-1 cells, treated with GM-CSF (25 ng/ml) for the indicated times, using Phospho-Stat5 (Tyr694) Antibody (upper) or Stat5 antibody (lower).
Flow Cytometry
Flow cytometric analysis of K562 cells, untreated (green) or STI571-treated (blue), using Phospho-Stat5 (Tyr694) Antibody.
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 BaF3 cells starved of IL-3 for 6 hours followed by induction with IL-3 for 45 minutes, and either 20 μl of Phospho-Stat5 (Tyr694) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse CIS Intron 1 Primers #5131, mouse SOCS-3 promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).
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Gouilleux, F. et al. (1994) EMBO J. 13, 4361-4369.
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Wakao, H. et al. (1994) EMBO J. 13, 2182-2191.
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Okutani, Y. et al. (2001) Oncogene 20, 6643-6650.
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Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855-25861.
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Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
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Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937-6944.
- Fata, J. E. et al. (2000) The osteoclast differentiation factor osteoprotegerin-ligand is essential for mammary gland development. Cell 103, 41-50. Applications: IHC-P (paraffin)
- Kapur, R. and Zhang, L. (2000) A novel mechanism of cooperation between c-Kit and erythropoietin receptor: Stem cell factor induces the expression of Stat5 and erythropoietin receptor resulting in efficient proliferation and survival by erythropoietin. J. Biol. Chem. 276, 1099-1106. Applications: Western Blotting
- Siren, A. L. et al. (2001) Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress. Proc. Natl. Acad. Sci. USA 98, 4044-4049. Applications: Western Blotting
- Reuther, G. W. et al. (2000) Identification and characterization of an activating TrkA deletion mutation in acute myeloid leukemia. Mol. Cell. Biol. 20, 8655-8666. Applications: Western Blotting
- Bergonzini, V. et al. (2004) HIV-Tat promotes cellular proliferation and inhibits NGF-induced differentiation through mechanisms involving Id1 regulation. Oncogene 23, 7701-7711. Applications: Western Blotting
- Brizzi, M. F. et al. (2002) Diabetic LDL inhibits cell-cycle progression via STAT5B and p21waf. J. Clin. Invest. 109 (1), 111. Applications: IC-IF IHC-P (paraffin) Western Blotting
- Hutt, J. A. et al. (2000) Signal transducer and activator of transcription 3 activates CCAAT enhancer-binding protein delta gene transcription in G0 growth-arrested mouse mammary epithelial cells and in involuting mouse mammary gland. J. Biol. Chem. 275, 29123-29131. Applications: Western Blotting
- Weisberg, E. et al. (2011) PLoS One 6, e25351. Applications: Western Blotting
- Menon, MP. et al. (2006) Blood 107, 2662-72. Applications: Western Blotting
- Nishioka, Y. et al.(2008) Blood 111, 5086-92. Applications: Flow
PhosphoSitePlus®：
STAT5A