Antibody Sampler Kits
GSK3 Antibody Sampler Kit |
イイネ！(0) | 
|
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
|
|---|---|---|---|---|
| #9369S | 1 Kit | 80,000 | ログインすると国内在庫状況がご確認いただけます。
|
|
9369 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
|---|
- 4337:
- Immunoprecipitation Western Blotting
- 9315:
- IHC / Paraffin Immunoprecipitation Western Blotting
- 9316:
- IHC / Paraffin Western Blotting
- 9323:
- IHC / Paraffin Immunofluorescence Western Blotting
- 9327:
- Immunoprecipitation Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
|---|---|---|---|---|---|
| GSK-3α (D80E6) Rabbit mAb #4337 | 40 µl | W IP | H M R Hm Mk | 51 | Rabbit IgG |
| Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb #9323 | 40 µl | W IHC-P IF-IC | H M R Mk | 46 | Rabbit IgG |
| Phospho-GSK-3α/β (Ser21/9) (37F11) Rabbit mAb (GSK-3α Preferred) #9327 | 40 µl | W IP | H M R Mk | 51, 46 | Rabbit IgG |
| GSK-3β (27C10) Rabbit mAb #9315 | 40 µl | W IP IHC-P | H M R Mk | 46 | Rabbit IgG |
| Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316 | 40 µl | W IHC-P | H M R Mk | 51 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
| 貯法 | |
|---|---|
| -20℃ |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
|---|
Western Blotting
Western blot analysis of extracts from various cell types using GSK-3α (D80E6) Rabbit mAb #4337.
Western Blotting
Western blot analysis of extracts from various cell lines using GSK-3β (27C10) Rabbit mAb #9315.
Western Blotting
Western blot analysis of extracts from COS-7 cells, λ-phosphatase or PDGF-treated, using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316 (upper) or GSK-3α Antibody #9338 (lower).
Western Blotting
Western blot analysis of extracts from wild type (lanes 1,2), GSK-3α (-/-) (lanes 3,4) or GSK-3β (-/-) (lanes 5,6) mouse embryonic fibroblasts (MEFs), untreated or PDGF treated, using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb #9323 (upper) and GSK-3α/β Antibody (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).
Western Blotting
Western blot analysis of extracts from COS-7 cells, λ-phosphatase or PDGF-treated, using Phospho-GSK-3α/β (Ser21/9) (37F11) Rabbit mAb (GSK-3α Preferred) #9327 (upper) or GSK-3α Antibody #9338 (lower).
| バックグラウンド |
|---|
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
| 使用例 | |
|---|---|
| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。