Tyrosine Kinases / Adaptors
T Cell Signaling Antibody Sampler Kit |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #9382S | 1 Kit | 103,000 | ログインすると国内在庫状況がご確認いただけます。
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9382 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 2717:
- Western Blotting
- 2751:
- Immunoprecipitation Western Blotting
- 2787:
- Flow Immunofluorescence Western Blotting
- 3165:
- Flow Immunofluorescence Immunoprecipitation Western Blotting
- 3584:
- Immunoprecipitation Western Blotting
- 4958:
- IHC / Paraffin Immunoprecipitation Western Blotting
- 9166:
- Flow IHC / Paraffin Immunoprecipitation Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
|---|---|---|---|---|---|
| Zap-70 (D1C10E) XP® Rabbit mAb #3165 | 40 µl | W IP IF-F F | H M | 70 | Rabbit IgG |
| Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb #2717 | 40 µl | W | H M | 70, 72 kDa | Rabbit IgG |
| SLP-76 Antibody #4958 | 40 µl | W IP IHC-P | H M | 76 | Rabbit |
| Phospho-LAT (Tyr191) Antibody #3584 | 40 µl | W IP | H | 36, 38 | Rabbit |
| LAT Antibody #9166 | 40 µl | W IP IHC-P F | H M (R) | 36, 38 | Rabbit |
| Phospho-Lck (Tyr505) Antibody #2751 | 40 µl | W IP | H M | 56 | Rabbit |
| Lck (73A5) Rabbit mAb #2787 | 40 µl | W IF-IC F | H | 56 KDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
| 貯法 | |
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| -20℃ |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from Jurkat cells treated with with either H2O2 (2mM for 2 minutes) or with λ phosphatase; and extracts from Ramos cells treated with anti-human IgM (12 μg/ml for 2 minutes) using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb #2717.
Western Blotting
Western blot analysis of extracts from Jurkat cells (serum starved) treated with calf intestinal alkaline phosphatase (CIP) or H2O2 (2 mM), using Phospho-Lck (Tyr505) Antibody #2751 (upper) or total Lck Antibody #2752 (lower).
Western Blotting
Western blot analysis of Jurkat cell lysates, using Lck (73A5) Rabbit mAb #2787.
Western Blotting
Western blot analysis of extracts from Jurkat cells using Zap-70 (D1C10E) XP® Rabbit mAb #3165.
Western Blotting
Western blot analysis of extracts from serum starved Jurkat cells, either untreated or anti-CD3 treated (10 µg/ml for 2 minutes), using Phospho-LAT (Tyr191) Antibody #3584.
Western Blotting
Western blot analysis of extracts from Jurkat and BW5147cells, using SLP-76 Antibody #4958.
Western Blotting
Western blot analysis of extracts from Jurkat cells, either untreated or treated with λ phosphatase, using LAT Antibody #9166.
| バックグラウンド |
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The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling (9). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (10,11). The phosphorylation of LAT at Tyr171 and 191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (11-12).
- Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
- Iwashima, M. et al. (1994) Science 263, 1136-1139.
- Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
- Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
- Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
- Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
- Wiestner, A. et al. (2003) Blood 101, 4944-4951.
- Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.
- Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-83.
- Zhang, W. et al. (1998) Cell 92, 83-92.
- Paz, P.E. et al. (2001) Biochem J 356, 461-71.
- Zhang, W. et al. (2000) J Biol Chem 275, 23355-61.
| 使用例 | |
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本製品は試験研究用です。