Chromatin Regulation / Acetylation
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月7日15時25分 現在 |
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| #9441S | 100 μL | 53,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9441L | 300 μL | 127,000 |
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9441 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 9441:
- ChIP Agarose ChIP Magnetic ELISA Peptide IHC / Paraffin Immunofluorescence Immunoprecipitation Western Blotting
下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:100)、免疫組織染色 (パラフィン) (1:1,600)、免疫蛍光細胞染色 (IF-IC) (1:200)、ELISA-P (1:1,000)、ChIP (1:50) |
| 種交差性 | |
|---|---|
| すべての種 |
| 特異性・感度 | |
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| リジン残基のε-アミノ基がアセチル化により翻訳後修飾されたタンパク質を検出します。様々な配列のアセチル化リジンを認識します。このことはアセチル化Histone、p53、CBP、PCAF、および化学的にアセチル化させたBSAの検証で実証されています。また、アセチル化していないBSAでは25 μg までは認識しないのに対し、化学的にアセチル化したBSAではわずか0.04 ng で反応が見られました。 |
| 使用抗原 | |
|---|---|
| アセチル化リジンを含む合成ペプチド |
| モチーフ | |
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| XXX(Kac)XXX |
| 抗体の由来 | |
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| ウサギ |
| 貯法 | |
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| -20℃ |
| License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact at info@cstj.co.jp. |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or sodium butyrate-treated (5 mM for 24 hours), showing an increase in histone acetylation using Acetylated-Lysine Antibody.
Western Blotting
Specificity and sensitivity of Acetylated-Lysine Antibody assayed on acetylated BSA (4; 1; 0.2; 0.04 or 0.008 ng in lanes 1-5) or nonacetylated BSA (25,000; 5,000; 1,000 or 200 ng in lanes 6-9).
Western Blotting
Western blot analysis of extracts from COS cells, untreated or TSA-treated, grown in 10% FBS (lanes 1 and 2) or serum starved for 18 hours (lanes 3 and 4), using Acetylated-Lysine Antibody (upper) or p44/42 MAP Kinase Antibody #9102 (lower).
IP
Western blot analysis of immunoprecipitated p53 showing an increase in p53 acetylation using Acetylated-Lysine Antibody (upper) or p53 antibody (lower). p53 was immunoprecipitated from lysates from 293 cells, untreated or UV-treated, using p53 Antibody #9282.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetylated-Lysine Antibody.
IHC-P (paraffin)
Immunohistochemical staining of a paraffin-embedded human breast tumor section showing nuclear and cytoplasmic localization of proteins with acetylated lysine residues using Acetylated-Lysine Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded NIH/3T3 untreated (left) or TSA-treated (right) using Acetylated-Lysine Antibody.
IF-IC
Confocal immunofluorescent analysis of NIH/3T3 cells, untreated (left) or SAHA-treated (right), labeled with Acetylated-Lysine Antibody (green). Actin filaments have been labeled with Alexa Fluor R 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 20 μl of Acetylated-Lysine Antibody or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) #9002. The enriched DNA was quantified by real-time PCR, using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
| バックグラウンド |
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Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10).
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| 使用文献 |
|---|
- Werner, H.B. et al. (2007) J Neurosci 27, 7717-30. Applications: Western Blotting
- Yuan, Z.L. et al. (2005) Science 307, 269-73. Applications: Western Blotting
- Hu, J. and Colburn, N.H. (2005) Mol Cancer Res 3, 100-9. Applications: Western Blotting
- Kurahashi, T. et al. (2005) Mol Biol Cell 16, 4705-13. Applications: Western Blotting
- Wang, R. et al. (2005) J Biol Chem 280, 11528-34. Applications: Western Blotting
- Chen, L. et al. (2005) Mol Cancer Ther 4, 1311-9. Applications: Western Blotting
- Zheng, G. and Yang, Y.C. (2005) J Biol Chem 280, 40773-81. Applications: Western Blotting
- Qiu, Y. et al. (2004) J Biol Chem 279, 9796-802. Applications: IP
- Ard, P.G. et al. (2002) Mol Cell Biol 22, 5650-61. Applications: IP
- Yu, X. et al. (2002) J Natl Cancer Inst 94, 504-13. Applications: Western Blotting
- Bereshchenko, O.R. et al. (2002) Nat Genet 32, 606-13. Applications: Western Blotting
- Chen Lf et al. (2001) Science 293, 1653-7. Applications: Western Blotting
- Mal, A. et al. (2001) EMBO J 20, 1739-53. Applications: Western Blot
- Sano, Y. and Ishii, S. (2001) J Biol Chem 276, 3674-82. Applications: Western Blotting
- Chan, H.M. et al. (2001) Nat Cell Biol 3, 667-74. Applications: Western Blot
- Fu, M. et al. (2000) J Biol Chem 275, 20853-60. Applications: IP
- Li, S. et al. (2000) Proc Natl Acad Sci U S A 97, 7166-71. Applications: Western Blot
- Martínez-Balbás, M.A. et al. (2000) EMBO J 19, 662-71. Applications: Immunoprecipitation
本製品は試験研究用です。