TGF-beta/Smad Signaling
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| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年7月30日15時15分 現在 |
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| #9511S | 100 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9511L | 300 μL | 137,000 |
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推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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| 用途（希釈倍率） | |
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| ウェスタンブロッティング（1:1,000）、フローサイトメトリー（1:100）、ChIP（1:100） | |
| 種交差性 | |
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| ヒト、マウス、ラット、ミンク、アフリカツメガエル | |
| 特異性・感度 | |
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| 内在性レベルのSer463/465 がリン酸化されたSmad1 タンパク質および同様の部位がリン酸化されたSmad5、Smad8 タンパク質を検出します。他のSmad 関連タンパク質とは交差しません。 | |
| 検出タンパク質の分子量 | |
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| 60 kDa | |
| 使用抗原 | |
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| ヒトのSmad5 タンパク質のSer463/465 周辺領域（合成リン酸化ペプチド） | |
| 抗体の由来 | |
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| ウサギ | |
| 貯法 | |
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| -20℃ | |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from untransfected Xenopus cells and BMPRI/Smad1-transfected 293 cells, untreated or BMP-4-treated, using Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad8 (Ser426/428) Antibody. (Xenopus cells provided by Dr. Malcolm Whitman, Harvard University, Massachusetts.)
Flow Cytometry
Flow cytometric analysis of HeLa cells, untreated (blue) or BMP-treated (green), using Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad8 (Ser426/428) Antibody compared with a nonspecific negative control antibody (red).
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 MCF-7 cells treated with Human BMP2 #4697 (50 ng/ml) for 1 h and either 10 μl of Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad8 (Ser426/428) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP™ Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP™ Human ID1 Promoter Primers #5139, human SMAD6 promoter primers, and SimpleChIP™ Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
| バックグラウンド |
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Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).
| 使用文献 |
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- Kyung, A. et al. (2001) BMPR-1A signaling is required for the formation of the apical ectodermal ridge and dorsal-ventral patterning of the limb. Development 128, 4449-4461. Applications: IHC-Paraffin
- Seto, H. et al. (2004) Distinct roles of Smad pathways and p38 pathways in cartilage-specific gene expression in synovial fibroblasts. J. Clin. Invest. 113, 718-726. Applications: Western Blotting
- Jackson, L. F. et al. (2003) Defective valvulogenesis in HB-EGF and TACE-null mice is associated with aberrant BMP signaling. EMBO J. 22, 2704-2716. Applications: IHC-P (paraffin)
- Watabe, T. et al. (2003) TGF-beta receptor kinase inhibitor enhances growth and integrity of embryonic stem cell-derived endothelial cells. J. Cell Biol. 163, 1303-1311. Applications: Western Blotting
- Nagata, M. et al. (2006) Genes Cells 11, 1267-80. Applications: IF-IC (In Cells) Western Blotting
- Kondo, M. et al. (2004) Cell Death Differ 11, 1092-101. Applications: Western Blotting
本製品は試験研究用です。
