Apoptosis and Autophagy
- Apoptosis and Autophagy
PhosphoSitePlus®：
PARP1
- 推奨プロトコール
Cleaved PARP (Asp214) Antibody (Human Specific) |
イイネ！(3) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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|---|---|---|---|---|
| #9541S | 100 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9541L | 300 μL | 137,000 |
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9541 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫組織染色 (パラフィン) (1:50)、免疫蛍光細胞染色 (IF-IC) (1:400)、フローサイトメトリー (1:100) |
| 種交差性 | |
|---|---|
| ヒト |
| 特異性・感度 | |
|---|---|
| 内在性レベルの開裂したPARP1 タンパク質の大断片 (89kDa) を検出します。全長PARP1 タンパク質あるいは他のPARP アイソフォームは検出しません。 |
| 検出タンパク質の分子量 | |
|---|---|
| 89 kDa |
| 使用抗原 | |
|---|---|
| ヒトのPARP タンパク質のAsp214 周辺のC末端領域 (合成ペプチド) |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of Jurkat cells, untreated or etoposide-treated (25 µM, 5hrs), using Cleaved PARP (Asp214) Antibody (Human Specific).
IHC-P (paraffin)
Immunohistochemical staining of paraffin-embedded human tonsil, showing nuclear localization, using Cleaved PARP (Asp214) Antibody (Human Specific).
IHC-P (paraffin)
Immunohistochemical analysis using Cleaved PARP (Asp214) Antibody (Human Specific) on SignalSlide™ Cleaved Caspase-3 IHC Controls #8104 (paraffin-embedded Jurkat cells, untreated (left) or etoposide-treated (right)).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved PARP (Asp214) Antibody (Human Specific) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine #9953 treated (right) labeled with Cleaved PARP (Asp214) Antibody (Human Specific) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
| バックグラウンド |
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PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
- Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
- Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
- Cohen, G.M. (1997) Biochem. J. 326, 1-16.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
- Tewari, M. et al. (1995) Cell 81, 801-809.
- Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.
| 使用文献 |
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- Brunet, A. et al. (2001) Protein kinase SGK mediates survival signals by phosphorylating the Forkhead transcription factor FKHRL1 (FOXO3a). Mol. Cell. Biol. 21, 952-965. Applications: Western Blotting
- Bhakar, A. L. et al. (2003) Apoptosis induced by p75NTR overexpression requires Jun kinase-dependent phosphorylation of Bad. J. Neurosci. 23, 11373-11381. Applications: Western Blotting
- Jiang, C. et al. (2001) Caspases as key executors of methyl selenium-induced apoptosis (anoikis) of DU-145 prostate cancer cells. Cancer Res. 61, 3062-3070. Applications: Western Blotting
本製品は試験研究用です。